Investigation of the Mechanism of Pachyman against Gout Arthritis with Network Pharmacology Analysis and Verification In Vivo.

Purpose: Pachyman, derived from Poria cocos, has been used to treat gouty arthritis (GA) for thousands of years, although its precise role and mechanisms remain unclear. Herein, we investigate the therapeutic effects of pachyman on GA and explore their underlying mechanisms.

Methods: Network pharmacology and experimental methods were employed to investigate the therapeutic mechanisms of pachyman against GA.

A chromosome-level genome assembly of skipjack tuna, Katsuwonus pelamis (Perciformes: Scombridae).

Skipjack tuna (Katsuwonus pelamis), a highly migratory pelagic species widely distributed in tropical and subtropical oceanic regions, has consistently ranked third in global fishery landings from 2015 to 2022 and holds substantial economic significance for the coastal fisheries of Pacific Rim countries. Integrating PacBio and Hi-C data, a chromosome-level assembly of its genome was accomplished. This assembly comprises 24 pseudo-chromosomes, yielding a genome size of 827.

Assessing the trophic interactions between two pelagic fishes in the northwestern Pacific based on stable isotopes.

Scomber japonicus (S. japonicus) and Sardinops melanostictus (S. melanostictus) are widely distributed off the coast of Japan and in the northwestern Pacific.

Diversity but not abundance of ingested plastics changes with ontogenetic dietary shift: Stable isotope insights into plastic contamination in a mesopelagic predator longnose lancetfish Alepisaurus ferox.

We employed Fourier infrared spectroscopy, laser infrared technology, and stable isotope analysis to investigate the relationships between characteristics of ingested plastics and size-related feeding ecology of a mesopelagic predator, longnose lancetfish Alepisaurus ferox. Plastics were detected in 81.48 % of specimens, up to 8.

A comparative study of the efficiency of mitochondria-targeted antioxidants MitoTEMPO and SKQ1 under oxidative stress.

MitoTEMPO (MT) and Visomitin (SKQ1) are regareded as mitochondria-targeted antioxidants, which inhibit production of mitochondrial reactive oxygen species (ROS). However, the differences in function between MT and SKQ1 remain unexplored. Herein, we investigated the differential potency of MT and SKQ1 in mitigating oxidative stress under different conditions.

Thrombospondin 1 and Reelin act through Vldlr to regulate cardiac growth and repair.

Adult mammalian cardiomyocytes have minimal cell cycle capacity, which leads to poor regeneration after cardiac injury such as myocardial infarction. Many positive regulators of cardiomyocyte cell cycle and cardioprotective signals have been identified, but extracellular signals that suppress cardiomyocyte proliferation are poorly understood. We profiled receptors enriched in postnatal cardiomyocytes, and found that very-low-density-lipoprotein receptor (Vldlr) inhibits neonatal cardiomyocyte cell cycle.

Application of Gradient-Dependent Optimal Interpolation in Fishery Analysis of Neon Flying Squid () in the Kuroshio-Oyashio Confluence Region.

A key issue in fishery forecasting is the collection of high-precision subsurface environmental data. A data assimilation method, named gradient-dependent optimal interpolation, was used to construct the near-real-time vertical temperature and salinity structure of a squid fishery ground based on Argo observations. The results were verified by truth-finding comparisons and applied to analyze the relationship between neon flying squid and the subsurface environment in the Kuroshio-Oyashio Confluence Region.

Genome-based reclassification of Hussain . 2016 as a later heterotypic synonym of Cha . 2014.

YIM F302 was compared with N5 to examine the taxonomic relationship between the two type strains. The 16S rRNA gene sequence of YIM F302 showed high similarity (99.9 %) to that of N5.

Fusing Local Shallow Features and Global Deep Features to Identify Beaks.

Cephalopods are an essential component of marine ecosystems, which are of great significance for the development of marine resources, ecological balance, and human food supply. At the same time, the preservation of cephalopod resources and the promotion of sustainable utilization also require attention. Many studies on the classification of cephalopods focus on the analysis of their beaks.

Global squid contamination by halogenated polycyclic aromatic hydrocarbons and its trade induced risk transfer.

Squid is traded globally as an important food resource. However, the occurrence of carcinogenic halogenated polycyclic aromatic hydrocarbons (HPAHs) in squid and the risk of their transfer through trade is little understood or recognized. Here, we comprehensively evaluated the occurrence and risk transfer by quantifying the congener-specific concentrations of HPAHs in 121 squid samples collected from the Pacific, Atlantic and Indian oceans.

Feeding Strategies and Trophic Niche Divergence of Three Groups of Dosidicus gigas off Peru: Based on Stable Carbon and Nitrogen Isotopes and Morphology of Feeding Apparatuses.

Dosidicus gigas (D. gigas) is a pelagic cephalopod of ecological and economic importance widely distributed in the eastern Pacific Ocean. Generally, small-, medium-, and large-sized groups of the squids have been respectively identified on the basis of the mantle length (ML) of adults.

Corrigendum: The significance of cephalopod beaks as a research tool: An update.

[This corrects the article DOI: 10.3389/fphys.2022.

sp. nov., a marine bacterium isolated from deep seawater of the Mariana Trench.

A Gram-stain-negative, non-motile and rod-shaped bacterium, designated as strain W52, was isolated from deep seawater of the Mariana Trench and characterized phylogenetically and phenotypically. The strain could grow at 10-47 °C (optimum 32 °C), at pH 5.0-8.

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome.

Mitochondria, being the powerhouses of the cell, play important roles in bioenergetics, free radical generation, calcium homeostasis, and apoptosis. Mitophagy is the primary mechanism of mitochondrial quality control and is generally studied using microscopic observation, however in vivo mitophagy assays are difficult to perform. Evaluating mitophagy by imaging live organelles is an alternative and necessary method for mitochondrial research.

The significance of cephalopod beaks as a research tool: An update.

The use of cephalopod beaks in ecological and population dynamics studies has allowed major advances of our knowledge on the role of cephalopods in marine ecosystems in the last 60 years. Since the 1960's, with the pioneering research by Malcolm Clarke and colleagues, cephalopod beaks (also named jaws or mandibles) have been described to species level and their measurements have been shown to be related to cephalopod body size and mass, which permitted important information to be obtained on numerous biological and ecological aspects of cephalopods in marine ecosystems. In the last decade, a range of new techniques has been applied to cephalopod beaks, permitting new kinds of insight into cephalopod biology and ecology.

Pseudomonas marianensis sp. nov., a marine bacterium isolated from deep-sea sediments of the Mariana Trench.

A novel marine Gram-stain-negative, aerobic, rod-shaped bacterium, designated as strain PS1, was isolated from the deep-sea sediments of the Mariana Trench and characterized phylogenetically and phenotypically. Bacterial optimal growth occurred at 35 °C (ranging 10-45 °C), pH 6 (ranging pH 5-10) and with 11% (w/v) NaCl (ranging 0-17%). The 16S rRNA gene sequence similarity results revealed that strain PS1 was most closely related to Pseudomonas stutzeri ATCC 17588, Pseudomonas nitrititolerans GL14, Pseudomonas zhaodongensis NEAU-ST5-21, Pseudomonas xanthomarina DSM 18231 and Pseudomonas kunmingensis HL22-2 with 98.

Inhibition of mitochondrial superoxide promotes the development of hiPS-CMs during differentiation.

The redox state is a crucial determinant of the maturation transition of cardiomyocytes in vivo. Mitochondria, the primary site of superoxide generation, are very sensitive to various stimulations, including oxygen and nutrient supply. How mitochondrial superoxide affects the differentiation and development of induced pluripotent stem cell (iPSC)-derived cardiac myocytes (iPS-CMs) is not completely clear.

PGC-1-Mediated Mitochondrial Quality Control: Molecular Mechanisms and Implications for Heart Failure.

Mitochondria with structural and functional integrity are essential for maintaining mitochondrial function and cardiac homeostasis. It is involved in the pathogenesis of many diseases. Peroxisome proliferator-activated receptor coactivator 1 (PGC-1), acted as a transcriptional cofactor, is abundant in the heart, which modulates mitochondrial biogenesis and mitochondrial dynamics and mitophagy to sustain a steady-state of mitochondria.

Mitochondrial autophagy: molecular mechanisms and implications for cardiovascular disease.

Mitochondria are highly dynamic organelles that participate in ATP generation and involve calcium homeostasis, oxidative stress response, and apoptosis. Dysfunctional or damaged mitochondria could cause serious consequences even lead to cell death. Therefore, maintaining the homeostasis of mitochondria is critical for cellular functions.

[Composition and distribution of zooplankton species in the subtropical areas of the Northwestern Pacific Ocean].

Based on the investigation data of 44 stations in the area 28°－35° N, 147°－154° E of the Northwestern Pacific Ocean by the research ship 'Songhang' in March 2019, we analyzed species composition and distribution of zooplankton to understand zooplankton community structure in the subtropical areas of the Northwestern Pacific Ocean. The results showed that a total of 456 zooplankton species (including planktonic larvae and unidentified species) were recorded in the surveyed area, which were belonged to 8 categories and 14 groups. There were 163 species of copepods, as the dominant group.

[Preliminary analysis on the composition of fish and cephalopod in diet of in the high sea of Eastern Pacific Ocean].

is widely distributed in the Eastern Pacific Ocean and plays an important role in the marine ecosystem. In this study, we identified and analyzed 4131 otoliths and 75 beaks from the stomach content residuals of 62 samples, which were collected by Chinese squid jigging vessels in the high sea of Eastern Pacific Ocean from June to December 2019. The results showed that the preys of included 10 fish species and 4 cephalopod species.