The complete mitochondrial genome of the lobe coral (Anthozoa: Scleractinia) sequenced using ezRAD.

The mitochondrial genome of the coral was sequenced using ezRAD. The assembled genome consists of 18,647 bp, including 13 protein-coding genes, two ribosomal RNA genes and two transfer RNA genes. The gene arrangement was consistent with other scleractinian coral mitochondrial genomes.

MALDI imaging-based classification of hepatocellular carcinoma and non-malignant lesions in fibrotic liver tissue.

Histopathologic differentiation of nodular lesions in cirrhotic liver is difficult even for experienced hepatopathologists especially regarding diagnosis of hepatocellular carcinoma (HCC) in biopsies. For this reason, new tissue markers are needed to reinforce histopathologic decision-making. With advances in molecular techniques, proteomic analysis may help to confirm the diagnosis of HCC.

Discrimination and classification of liver cancer cells and proliferation states by Raman spectroscopic imaging.

Discrimination of nodular lesions in cirrhotic liver is a challenge in the histopathologic diagnostics. For this reason, there is an urgent need for new detection methods to improve the accuracy of the diagnosis of liver cancer. Raman imaging allows to determine the spatial distribution of a variety of molecules in cells or tissue label-free and to correlate this molecular information with the morphological structures at the same sample location.

Classification and prediction of HCC tissues by Raman imaging with identification of fatty acids as potential lipid biomarkers.

Purpose: Patients with hepatocellular carcinoma (HCC) can only be treated curatively at early stages and then have a favorable prognosis of this often fatal disease. For this reason, an early detection and diagnostic confirmation are crucial. Raman imaging spectroscopy is a promising technology for high-resolution visualization of the spatial distribution of molecular composition in tissue sections.

Classification of inflammatory bowel diseases by means of Raman spectroscopic imaging of epithelium cells.

We report on a Raman microspectroscopic characterization of the inflammatory bowel diseases (IBD) Crohn's disease (CD) and ulcerative colitis (UC). Therefore, Raman maps of human colon tissue sections were analyzed by utilizing innovative chemometric approaches. First, support vector machines were applied to highlight the tissue morphology (=Raman spectroscopic histopathology).

Nonlinear microscopy, infrared, and Raman microspectroscopy for brain tumor analysis.

Contemporary brain tumor research focuses on two challenges: First, tumor typing and grading by analyzing excised tissue is of utmost importance for choosing a therapy. Second, for prognostication the tumor has to be removed as completely as possible. Nowadays, histopathology of excised tissue using haematoxylin-eosine staining is the gold standard for the definitive diagnosis of surgical pathology specimens.

Surveillance strategies in inflammatory bowel disease.

Patients with longstanding ulcerative colitis (UC) are at increased risk of developing colorectal cancer (CRC). Although data for CRC risk in Crohn's disease (CD) are limited, it has been suggested that the risk is comparable to UC. Current strategies for the prevention and early detection of cancer in this high risk population are based on the concept of an inflammation-neoplasia-carcinoma sequence.

Malignant transformation in inflammatory bowel disease - surveillance guide.

Nowadays, it is considered as an established fact that patients with long-standing ulcerative colitis (UC) are at an increased risk of developing colorectal cancer (CRC). Although data for CRC risk in Crohn's disease (CD) are not as extensive, it has been suggested that the risks are comparable to UC. Current strategies for the prevention and early detection of cancer in this high-risk population are grounded in the concept of an inflammation-neoplasia-carcinoma sequence.

Exaggerated inflammatory response of primary human myeloid dendritic cells to lipopolysaccharide in patients with inflammatory bowel disease.

Inflammatory bowel disease (IBD) results from a breakdown of tolerance towards the indigenous flora in genetically susceptible hosts. Failure of dendritic cells (DC) to interpret molecular microbial patterns appropriately when directing innate and adaptive immune responses is conceivable. Primary (conventional, non-monocyte generated) CD1c(+)CD11c(+)CD14(-)CD16(-)CD19(-) myeloid blood or mucosal dendritic cells (mDC) from 76 patients with Crohn's disease (CD) or ulcerative colitis (UC) in remission, during flare-ups (FU) and 76 healthy or non-IBD controls were analysed by fluorescence activated cell sorter (FACS) flow cytometry and real-time polymerase chain reaction.

A comparative Raman and CARS imaging study of colon tissue.

An experimental evaluation of the information content of two complimentary techniques, linear Raman and coherent anti-Stokes Raman scattering (CARS) microscopy, is presented. CARS is a nonlinear variant of Raman spectroscopy that enables rapid acquisition of images within seconds in combination with laser scanning microscopes. CARS images were recorded from thin colon tissue sections at 2850, 1660, 1450 and 1000 cm(-1) and compared with Raman images.

Changing nurses' pain assessment practice: a collaborative research utilization approach.

It is not uncommon for a decade to pass between the time a research problem is identified and the time that research-based solutions are translated into standards for care. This quasi-experimental study demonstrated the effectiveness of a collaborative research utilization model directed towards the transfer of specific research-based knowledge (pain assessment) into practice for the purpose of helping to solve pain management problems. At the same time, nurses who participated in the model significantly improved their competency in research utilization and their attitudes towards research when compared to a control group who did not participate in the model.

Image enhancement. Developing a program for perioperative nurses.

An image campaign focuses on the strengths of nurses to promote nursing, and in doing so, helps nurses rediscover the values and commitment that are at the heart of the profession. Nursing's image will change when nurses themselves change.