Publications by authors named "Bidya Shrestha"

Article Synopsis
  • - Dengue fever is a significant public health issue in Nepal, primarily transmitted by the Aedes aegypti mosquito, and this study aimed to assess dengue seropositivity and the bacterial diversity in these mosquitoes from affected regions.
  • - Conducted between June and November 2013, the study analyzed 221 serum samples for anti-dengue IgM and collected mosquito larvae to identify the bacterial communities present in A. aegypti.
  • - Results showed a 15.38% seropositivity rate for dengue and highlighted that gram-negative bacteria were the most commonly isolated, with Staphylococcus spp., Pseudomonas spp., and Acinetobacter spp. being the predominant strains found in the mosquito's gut.
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Methicillin-resistant Staphylococcus aureus (MRSA) carrying the important virulence determinant, Panton-Valentine leukocidin (PVL), is an emerging infectious pathogen associated with skin and soft tissue infections as well as life-threatening invasive diseases. In carrying out the first PVL prevalence study in Nepal, we screened 73 nosocomial isolates of S. aureus from 2 tertiary care Nepali hospitals and obtained an overall PVL-positivity rate of 35.

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Background: Apart from being a major cause of mortality, nosocomial infections due to Staphylococcus aureus have been imposing a burden on patients, hospitals and health care systems. The present study was designed to determine the prevalence of methicillin resistant S. aureus (MRSA) among nosocomial isolates along with their phenotypic characterization.

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Staphylococcus aureus (n=84) isolated from the nostrils of a healthy population from Kathmandu and from the infectious cases (n=100) from Tribhuvan University Teaching Hospital, Kathmandu, Nepal were tested from May 1996 to March 1997 in Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal by microbiological and chemical methods to find out their beta lactamase activity. Among the healthy population, in domiciliary conditions 21.4% of the isolates were found beta lactamase producers.

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The prevalence of hepatitis B surface antigen (HBsAg) was studied among 2,585 healthy Nepalese men, aged 16-50 years, who required medical check-ups for employment abroad. Serum samples, collected during July-September 1999, were tested for HBsAg using a third-generation ELISA kit. Of the 2,585 men, 24 (0.

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