A rapid and selective method for simultaneous determination of six toxic phenolic compounds in mainstream cigarette smoke using single-drop microextraction followed by liquid chromatography-tandem mass spectrometry.

Cigarette smoke contains several toxic phenolic compounds, measurements of which are essential from a public health standpoint. This article describes a simple and selective analytical method for quantitative determination of six toxic phenolic compounds (phenol, catechol, resorcinol, hydroquinone, o-cresol, and p-cresol) from mainstream cigarette smoke using single-drop microextraction in combination with liquid chromatography-tandem mass spectrometry. Single-drop microextraction was applied prior to analysis by liquid chromatography-tandem mass spectrometry for the extraction and preconcentration of target phenolic compounds from raw cigarette smoke extract.

Evaluating intra-genetic variants of DJ-1 among Parkinson's disease patients of eastern India.

Objectives: Mutations in the DJ-1 gene have been described in autosomal recessive Parkinson's disease (PD) of European ancestry, Ashkenazi Jews, and Afro-Caribbean patients. Up to date, there is a lack of information about the prevalence of DJ-1 mutations among Indian PD patients.

Materials And Methods: In this study, we examined for DJ-1 mutations in Eastern Indian PD patients.

Environmental and familial risk factors of Parkinsons disease: case-control study.

Background: While the cause of Parkinson's disease (PD) remains unknown, evidence suggests certain environmental factors, such as well water drinking, herbicides, pesticides exposure and neurotoxins, may trigger the chain of oxidative reactions culminating in the death of dopaminergic neurons in substantia nigra to cause Parkinsonism. To investigate the possible impact of environmental risk factors for idiopathic PD, a case-control study was performed in the Eastern India.

Methods: During the period from January 1st, 2006 and December 10th, 2009, 175 PD patients (140 men, 35 women) and 350 non-Parkinson age-sex matched controls were included in the study.

Absence of commonly reported leucine-rich repeat kinase 2 mutations in Eastern Indian Parkinson's disease patients.

Background: Pathogenic mutations in leucine-rich repeat kinase 2 (LRRK2; PARK8) encoding dardarin, implicated in patients with autosomal dominant and sporadic Parkinson's disease (PD) among different ethnic groups (Ashkenazi Jews, North African Arabs, Basques) might be of some help in diagnostic screening and genetic counseling.

Aim Of The Study: We investigated the seven common mutations spanning exons 31, 35, and 41 reported in the LRRK2 gene among Eastern Indian patients with PD.

Methods: Mutations R1441G, R1441C, R1441H, G2019S, Y1699C, I2020T, and I2012T were screened in 320 individuals (PD, 150 and controls, 170) by direct sequencing.

A novel spectrofluorimetric method for the ultra trace analysis of nitrite and nitrate in aqueous medium and its application to air, water, soil and forensic samples.

A highly sensitive and virtually specific method has been developed for the trace and ultra trace 5ngml(-1)-1mugml(-1) fluorimetric analysis of nitrite. The method is based on the quenching action of nitrite on the native fluorescence of murexide (ammonium purpurate) [lambda(ex)=349.0nm, lambda(em)=444.