Mucosal invasion by fusobacteria is a common feature of acute appendicitis in Germany, Russia, and China.

Background/aim: To investigate the geographic occurrence of mucosa-invading Fusobacteria in acute appendicitis.

Patients And Methods: Carnoy- and formalin-fixated appendices from Germany, Russia, and China were comparatively investigated. Bacteria were detected using fluorescent in situ hybridization.

[A method for assessing bacterial microflora by fluorescence in situ hybridization].

The use of current diagnostic methods in a pathologist's practice, including the assessment of the bacterial microflora by the molecular microbiological technique fluorescence in situ hybridization is important and promising. The detection of bacteria and changes in the microbial flora makes it possible to clarify the nature of found morphological changes and to expand the diagnostic value of a postmortem study.