Toxicity of paclobutrazol-based pesticide on Lactuca sativa L.: germination, seedling development, and DNA damage.

Paclobutrazol, a fungicide of the triazole class, is widely used as an inducer of early flowering and fruiting by inhibiting gibberellin formation. However, biological assays using model organisms to evaluate their cytogenotoxic and mutagenic potential are still scarce. Therefore, this study aimed to investigate the effects of the commercial product Cultar® 250 SC (CP) and the pure substance (PBZ) on the germination and initial seedling development of Lactuca sativa L.

Be prepared: how does discontinuous hydration in Tabebuia heterophylla seeds induce stress tolerance in seedlings?

Discontinuous hydration and dehydration (HD) cycles refer to controlled imbibition followed by dehydration before seed germination. Here, we investigated whether the level of imbibition before HD cycles affects the physiology of Tabebuia heterophylla seeds and seedlings. Seeds were imbibed for 10 h (T1; phase I of imbibition) or 35 h (T2; phase II), dehydrated, and progressively rehydrated one to four times (HD cycles).

Implications of converting native forest areas to agricultural systems on the dynamics of CO emission and carbon stock in a Cerrado soil, Brazil.

The conversion of native vegetation to agricultural areas leads to a natural process of carbon loss but these systems can stabilize in terms of carbon dynamics depending on the management and conversion time, presenting potential to both store and stabilize this carbon in the soil, resulting in lower soil respiration rates. In this context, this study aimed to investigate the effect of converting native Cerrado forest areas to agricultural systems with a forest planted with Eucalyptus camaldulensis and silvopastoral systems on the dynamics of CO emission and carbon stock at different soil depths. The experimental sites are located in the Midwest of Brazil, in the coordinates 20°22'31″ S and 51°24'12″ W.

New Perspective on the Use of α-Bisabolol for Weed Control.

The indiscriminate use of synthetic herbicides reduces its effectiveness. Bioherbicides produced with metabolites emerge as an alternative to managing weeds. We aimed to analyze the phytotoxic potential of the essential oil of (EOVA) and the α-bisabolol molecule, its main component.

Photochemical attributes determine the responses of plant species from different functional groups of ferruginous outcrops when grown in iron mining substrates.

Environments originating from banded iron formations, such as the canga , are important reference ecosystems for the recovery of degraded areas by mining. The objective of this work was to evaluate if the relationship between morphofunctional and photosynthetic attributes of native canga species from different functional group results in distinct responses when grown in iron mining tailings substrate. The experiment was carried out with species belonging to different functional groups: a widespread semi-deciduous tree-shrub, Myrcia splendens ; an endemic deciduous shrub, Jacaranda caroba ; and a nitrogen-fixing herbaceous species, Periandra mediterranea .

IBA and melatonin increase trigonelline and caffeine during the induction and initiation of adventitious roots in Coffea arabica L. cuttings.

Caffeine and trigonelline are found in Coffea arabica, and show antioxidant roles and growth and development functions. However, there are no reports on trigonelline and caffeine in relation to coffee rooting. The aim was to evaluate the impact of application of indole-3-butyric acid (IBA) and melatonin on caffeine and trigonelline at different stages of adventitious rooting in cuttings.

Relationship between osteopathic manipulative treatment of the temporomandibular joint, molar shim and the orthostatic position: A randomized, controlled and double blinded study.

This randomized, controlled, double-blinded study related the effect of osteopathic manipulative treatment (OMT) of the temporomandibular joint (TMJ) and the orthostatic posture using the molar shim (MS) as a postural adjustment factor. Twenty individuals classified with temporomandibular disorder (TMD) were randomly assigned to a treated group (TG, n = 10) and placebo (PG, n = 10). The independent variables were: MS and OMT of the TMJ.

Integrative signaling of hydrogen peroxide and gibberellin on Zn-mediated alleviation of thermodormancy in sorghum seeds.

Increasing global temperatures could result in decreasing crop production by decreasing seed germination in the field due to thermodormancy acquisition. Certain metals appear to modulate seed thermodormancy, although the exact mechanisms of that effect have not yet been elucidated. We report here the effects of Zn on the thermodormancy of sorghum seeds.

Fertilization assures mineral nutrition but does not overcome the effects of Fe accumulation in plants grown in iron ore tailings.

The rupture of Fundão dam was the biggest environmental disaster of the worlds' mining industry, dumping tons of iron ore tailings into the environment. Studies have shown that the Fundão dam's tailings are poor in nutrients and have high Fe and Mn concentration. In this context, our objective was to evaluate the growth performance of two native tree species (Bowdichia virgilioides and Dictyoloma vandellianum) in two treatments: fertilized soil and fertilized tailings.

Interoception and Emotion: A Potential Mechanism for Intervention With Manual Treatment.

Interoception is considered a perception pathway as important as the exteroceptive pathways for determining responses to maintain homeostasis. There is evidence about the influence of the interoception on emotional responses as these expressions are considered to be a combination of physical, environmental and individual beliefs. A large percentage of afferent fibers in the body are related to free nerve endings which, when stimulated, reach the insular cortex that participates in the process of emotions.

Soil CO emission and soil attributes associated with the microbiota of a sugarcane area in southern Brazil.

The spatial structure of soil CO emission (FCO) and soil attributes are affected by different factors in a highly complex way. In this context, this study aimed to characterize the spatial variability patterns of FCO and soil physical, chemical, and microbiological attributes in a sugarcane field area after reform activities. The study was conducted in an Oxisol with the measurement of FCO, soil temperature (Ts), and soil moisture (Ms) in a regular 90 × 90-m grid with 100 sampling points.

xCO2 temporal variability above Brazilian agroecosystems: A remote sensing approach.

Agriculture and soil management practices are closely related to CO emissions in crop fields. These practices directly interfere on the carbon dynamics between the land and atmosphere. In this study, we investigated the temporal variability of the column-averaged dry-air mole fraction of atmospheric CO (xCO), solar-induced chlorophyll fluorescence (SIF), and the normalized difference vegetation index (NDVI) in areas with the main agroecosystems in southern-central Brazil as a way to understand if and how crops cycle and agricultural management could be associated with the temporal variability of NDVI, SIF and xCO.

Inhibitory Tests as Assessment Tools for Somatic Dysfunctions: Mechanisms and Practical Applications.

The capital element in the field of osteopathy and several other manual therapy methods, is the somatic dysfunction (SD), a functional imbalance that can involve different tissues and mechanisms in its genesis and maintenance. The main challenges found in the clinical scope are to understand the interaction, hierarchy, and relevance of the SD. Several manual tests are available to functionally evaluate the SD, each one with its applicability to analyze the different parameters of the SD.

The Intraosseous Dysfunction in the Osteopathic Perspective: Mechanisms Implicating the Bone Tissue.

The somatic dysfunction (SD) is a protagonist in the context of theories and practices involving osteopathy and various other manual therapy methods. It is considered an obstacle to the body's inherent self-regulatory capabilities, and several tissues may be involved in this dysfunctional process, including the bone. The so-called intraosseous dysfunction refers to the restriction of natural flexibility of the fibrous components of the bone tissue matrix, or of the nonossified cartilaginous or membranous areas.

Does Samarco's spilled mud impair the growth of native trees of the Atlantic Rainforest?

The failure of the Fundão dam, the largest environmental disaster in the world's mining sector, was responsible for releasing millions of cubic meters of iron ore tailings into the environment. It affected thousands of hectares of the Atlantic Forest domain, one of the biodiversity hotspots for conservation. Considering the urgency to restore the flora of the affected area, we evaluated the effects that iron ore tailings from the Fundão reservoir have on the germination and initial growth of tree species native to the Atlantic Forest in the Rio Doce basin.

Does integrative effects of glyphosate, gibberellin and hydrogen peroxide ameliorate the deleterious effects of the herbicide on sorghum seed through its germination?

We investigated the interconnected roles of reactive oxygen species (ROS) generated upon seed exposure to glyphosate and/or gibberellic acid (GA), and the possible interaction between the herbicide and the plant hormone during germination of sorghum seeds. GA decreased antioxidant enzyme activity in embryos, and the over accumulation of hydrogen peroxide (HO) in 1000 mM GA-treated seeds resulted in the lowest germinability among treatments. The deleterious effects of glyphosate on germination rate, in contrast, were not related to HO accumulation, but to its interference with the mitochondrial electron transport chain.

Effects of Ciprofloxacin and Roundup on seed germination and root development of maize.

Their continuous release into the environment, associated with their inherent biological activity, has motivated investigations into the detrimental effects of antibiotics and herbicides in natural and agricultural ecosystems. In this study, the interactive effects of the antibiotic ciprofloxacin (Cipro) and the herbicide Roundup on seed germination and root development were investigated. Although both compounds act as inhibitors of the mitochondrial electron transport chain in seeds, neither Cipro nor Roundup disrupted germinability of maize seeds.

Therapeutic challenges in pregnant women with paroxysmal nocturnal hemoglobinuria: A case report.

Introduction: Paroxysmal nocturnal hemoglobinuria (PNH) is a rare acquired disease, arising from the mutation of clonal hematopoietic stem cells, with an estimated incidence of 1 to 5 cases per million individuals. In pregnant women, adequate information regarding the prevalence of PNH is lacking, and its management has been a challenge because of the significant complications in this group. The condition is diagnosed based on clinical findings and laboratory tests.

Ciprofloxacin vs. temperature: Antibiotic toxicity in the free-floating liverwort Ricciocarpus natans from a climate change perspective.

The physiological responses of the aquatic liverwort Ricciocarpus natans to ciprofloxacin (Cipro) exposure under different growth temperatures were investigated. Cipro appears to act as an inhibitor of mitochondrial Complex III by blocking the oxidation of quinol, resulting in the formation of hydrogen peroxide (HO). HO accumulation upon Cipro exposure is responsible for decreased photosynthesis in plants.

Glyphosate Can Decrease Germination of Glyphosate-Resistant Soybeans.

We investigated the effects of different concentrations of glyphosate acid and one of its formulations (Roundup) on seed germination of two glyphosate-resistant (GR) and one non-GR variety of soybean. As expected, the herbicide affected the shikimate pathway in non-GR seeds but not in GR seeds. We observed that glyphosate can disturb the mitochondrial electron transport chain, leading to HO accumulation in soybean seeds, which was, in turn, related to lower seed germination.

Ciprofloxacin induces oxidative stress in duckweed (Lemna minor L.): Implications for energy metabolism and antibiotic-uptake ability.

We investigate the physiological responses and antibiotic-uptake capacity of Lemna minor exposed to ciprofloxacin. Ciprofloxacin (Cipro) induced toxic effects and hormesis in plants by significantly modifying photosynthesis and respiration pathways. A toxic effect was induced by a concentration ≥1.

Integrative effects of zinc and temperature on germination in Dimorphandra wilsonii rizz.: Implications of climate changes.

The integrative effects of zinc (Zn; 0 mg L , 75 mg L , 150 mg L , and 200 mg L ) and temperature (25 °C, 30 °C, and 35 °C) on seed germination of the threatened Brazilian species Dimorphandra wilsonii were evaluated. Zinc effects on seed germination were only observed at 30 °C and 35 °C. By stimulating respiration rates, rising temperatures accentuate hydrogen peroxide (H O ) formation in germinating seeds in the presence of Zn.

Abscisic acid regulates seed germination of Vellozia species in response to temperature.

The relationship between the phytohormones, gibberellin (GA) and abscisic acid (ABA) and light and temperature on seed germination is still not well understood. We aimed to investigate the role of the ABA and GA on seed germination of Vellozia caruncularis, V. intermedia and V.

Effects of glyphosate acid and the glyphosate-commercial formulation (Roundup) on Dimorphandra wilsonii seed germination: Interference of seed respiratory metabolism.

Glyphosate-formulations are widely used in the Brazilian Cerrado (neotropical savanna) with little or no control, threatening population of the endangered species Dimorphandra wilsonii. We investigated the toxicity of different concentrations (0, 5, 25 and 50 mg l) of glyphosate acid and one of its formulations (Roundup) on seed germination in D. wilsonii.

Control of macaw palm seed germination by the gibberellin/abscisic acid balance.

The hormonal mechanisms involved in palm seed germination are not fully understood. To better understand how germination is regulated in Arecaceae, we used macaw palm (Acrocomia aculeata (Jacq.) Lodd.