Lysosome and phagosome stability in lethal cell injury. Morphologic tracer studies in cell injury due to inhibition of energy metabolism, immune cytolysis and photosensitization.

In two types of cell injury in a tissue culture system, the possibility was tested that lysosome rupture may be a lethal cellular reaction to injury, and thus an important general cause of irreversibility of damage in injured tissue. Prior labeling of secondary lysosomes with the fluorochrome acridine orange, or with ferritin, was used to trace changes in lysosomes after applying an injury. The metabolic inhibitors iodoacetate and cyanide were used together to block the cell's energy supply, or attachment of antiserum and subsequent complement attack were used to damage the surface membrane, producing rapid loss of cell volume control.

Ultrastructural features of Mycoplasma pneumoniae.

The ultrastructure of Mycoplasma pneumoniae cultivated in broth on glass and plastic surfaces was studied by scanning and transmission electron microscopy. The organisms grew as filaments, which by over-crossing eventually formed a dense network on the surface and in colonies composed mainly of rounded and elongated forms. The filaments were usually thinner at the ends and terminated with a knob-like structure.