Probing the binding mode and interactions of proteinase K and glutathione: molecular simulation and experiments.

Proteinase K, a serine protease from , is crucial in research due to its potent proteolytic activity, which relies on conformational stability and substrate affinity. Glutathione (GSH), an essential intracellular antioxidant, regulates various physiological processes by interacting with proteins, influencing their stability and function. Despite the importance of both proteinase K and GSH, their potential interaction remains unexplored.

An artificial intelligence handheld sensor for direct reading of nickel ion and ethylenediaminetetraacetic acid in food samples using ratiometric fluorescence cellulose paper microfluidic chip.

User-friendly in-field sensing protocol is crucial for the effective tracing of intended analytes under less-developed countries or resources-limited environments. Nevertheless, existing sensing strategies require professional technicians and expensive laboratory-based instrumentations, which are not capable for point-of-care on-site analyses. To address this issue, artificial intelligence handheld sensor has been designed for direct reading of Ni and EDTA in food samples.

Artificial intelligence-integrated smartphone-based handheld detection of fluoride ion by Al-triggered aggregation-induced red-emssion enhanced carbon dots.

Artificial intelligence (AI)-integrated smartphone-based handheld determination platform, based on 3D printed accessory, Al-triggered aggregation-induced red-emssion enhanced carbon dots (CDs) test strip, and smartphone with self-developed YOLO v3 AI algorithm-based application, proves the feasibility for intelligent real-time on-site quantitation of F through tracking a consecutive fluorescence (FL) colour change. CDs, manifesting dual emission of moderate green emission at 512 nm and weak red one at 620 nm under 365 nm excitation, were synthesized hydrothermally from alizarin carmine and citric acid. CDs@Al, with distinct aggregation-induced red-emssion enhancement and green-emssion quenchment, were prepared by adding Al to the CDs solution.

Novel [4 + 2]-cycloaddition of 1-phenyl-1-benzothiophenium salts with dienes. Experimental evidence for a lack of aromaticity in the thiophene ring.

The [4 + 2]-cycloaddition reaction of 1-phenyl-1-benzothiophenium triflates has been conducted for the first time. [4 + 2]-Cycloaddition with dienes such as cyclopentadiene and 1,3-diphenylisobenzofuran occurs successfully to give cycloadducts. This result indicates that the C=C bond of the thiophene ring acts as a 2pi electron component in the cycloaddition reaction.