SARS-CoV-2 surveillance-based on municipal solid waste leachate in Brazil.

Municipal solid waste leachate-based epidemiology is an alternative viral tracking tool that applies fresh truck leachate as an early warning of public health emergencies. This study aimed to investigate the potential of SARS-CoV-2 surveillance based on solid waste fresh truck leachate. Twenty truck leachate samples were ultracentrifugated, nucleic acid extracted, and real-time RT-qPCR SARS-CoV-2 N1/N2 applied.

A review on membrane concentrate management from landfill leachate treatment plants: The relevance of resource recovery to close the leachate treatment loop.

Membrane filtration processes have been used to treat landfill leachate. On the other hand, closing the leachate treatment loop and finding a final destination for landfill leachate membrane concentrate (LLMC) - residual stream of membrane systems - is challenging for landfill operators. The re-introduction of LLMC into the landfill is typical; however, this approach is critical as concentrate pollutants may accumulate in the leachate treatment facility.

Comparison between Aliivibrio fischeri and activated sludge microorganisms in the evaluation of the toxic pollutants of leachates from Brazilian landfills.

Ecotoxicological assessment of landfill leachate has become a priority to determine its impacts on the ecosystem. Toxicity assays with microorganisms stand out due to their quick response, low cost and ease of testing. In this context, the present study evaluated the acute toxic effects of leachates from two landfills of different ages and modes of operation to bacterium Aliivibrio fischeri and activated sludge microorganisms and the ammonia nitrogen and humic substances (HS) sensitivity to these organisms.

Nanofiltration applied to the landfill leachate treatment and preliminary cost estimation.

The leachate treatment by nanofiltration (NF) process has received much attention over the last two decades. Previous studies focused on the investigation of the technical feasibility of NF. However, there are a limited number of works that examined leachate treatment costs.

Cost estimation of landfill leachate treatment by reverse osmosis in a Brazilian landfill.

The reverse osmosis (RO) process has been increasingly applied to landfill leachate treatment. The published literature reports several studies that investigated the technical feasibility of RO. However, information about process costs is scarce.

Evaluation of toxic potential of leachate originating from experimental landfill cells containing household waste and healthcare waste.

Two experimental cells with household solid waste and healthcare solid waste were monitored in order to evaluate the pollution potential, and its toxicity effects corresponding to the chemical substances present in the leachates generated, correlating the physico-chemical composition with the ecotoxicity results (organisms and ). From the statistical evaluation of the physico-chemical analysis results, leachate generated in the household solid waste cell presented greater or equal values than to the healthcare solid waste cell, except for the turbidity parameter. The ecotoxicity results showed the same behaviour as that obtained with the physico-chemical analysis.

Evaluation of reduction estrogenic activity in the combined treatment of landfill leachate and sanitary sewage.

Combined treatment of urban solid waste leachate and domestic sewage has emerged in Brazil and worldwide as an alternative to minimize the difficulties of treating landfill leachate. Landfill leachate is a complex matrix of organic and inorganic substances, recalcitrant material and xenobiotic organic compounds that are considered to be highly toxic, as well as endocrine disrupting compounds (EDC) that contribute to increased estrogenic activity. Although combined treatment is feasible for removal of organic matter, ammonia and toxicity, the efficiency of the removal of estrogenic substance is poorly investigated.

Optimization of randomly amplified polymorphic DNA-polymerase chain reaction for molecular typing of Salmonella enterica serovar Typhi.

Optimization of the RAPD reaction for characterizing Salmonella enterica serovar Typhi strains was studied in order to ensure the reproducibility and the discriminatory power of this technique. Eight Salmonella serovar Typhi strains isolated from various regions in Brazil were examined for the fragment patterns produced using different concentrations of DNA template, primer, MgCl2 and Taq DNA polymerase. Using two different low stringency thermal cycle profiles, the RAPD fingerprints obtained were compared.