Publications by authors named "Bian Zhuan"

Non-syndromic orofacial clefts (NSOC) are common craniofacial birth defects, and result from both genetic and environmental factors. NSOC include three major sub-phenotypes: non-syndromic cleft lip with palate (NSCLP), non-syndromic cleft lip only (NSCLO) and non-syndromic cleft palate only (NSCPO), NSCLP and NSCLO are also sometimes grouped as non-syndromic cleft lip with or without cleft palate (NSCL/P) based on epidemiology. Currently known loci only explain a limited proportion of the heritability of NSOC.

View Article and Find Full Text PDF

Introduction: Odontoblasts, terminally differentiated dentin-producing cells, critically rely on lysosomal functions for intracellular recycling and renewal. Beyond their traditional degradative role, lysosomes actively orchestrate cellular responses to external stimuli through precise and rapid intracellular trafficking and positioning. This study aimed to explore the influence of lysosomal positioning on odontoblast mineralization and the underlying mechanisms implicated in carious inflammation.

View Article and Find Full Text PDF
Article Synopsis
  • - Tertiary lymphoid structures (TLS) are linked to various diseases, such as cancers and chronic infections, but their role in immune responses and formation processes is not completely understood.
  • - The dental pulp, with its unique immune cell organization and connection to the outside environment, provides a valuable model for studying TLS and immune responses to bacterial infections.
  • - Single-cell RNA sequencing and immunofluorescence staining in healthy versus inflamed dental pulp reveal markers and increased immune cell activity, indicating that TLS forms in inflamed conditions and may be driven by molecules like CCL3.
View Article and Find Full Text PDF
Article Synopsis
  • Only a small number of cancer patients benefit from immune checkpoint blockade therapy due to complex interactions among immune checkpoint pathways and molecules in circulating small extracellular vesicles (sEVs).
  • The study found that PD-1 and CD80 on immunocyte-derived sEVs lead to reduced PD-L1 on tumor cell membranes while increasing PD-L1 secretion, contributing to systemic immunosuppression and making tumors less responsive to immune attacks.
  • Analyzing multiple checkpoint molecules on circulating sEVs can help differentiate between patients who will respond well to anti-PD-1 treatments and those who will not, highlighting a potential target for improving cancer therapies.
View Article and Find Full Text PDF

Purpose: Exosomes are important "messengers" in cell-cell interactions, but their potential effects on palatal fusion are still unknown. This study aimed to explore the role and mechanism of exosomes derived from palatal mesenchymal cells in epithelial-mesenchymal communication during palatogenesis.

Methods: The expression of exosome marker CD63 and CD81 in palatal cells during palatogenesis was detected by immunofluorescence staining.

View Article and Find Full Text PDF

The dentine sialophosphoprotein (DSPP) gene is the only identified causative gene for dentinogenesis imperfecta type 2 (DGI-II), dentinogenesis imperfecta type 3 (DGI-III) and dentine dysplasia type 2 (DD-II). These three disorders may have similar molecular mechanisms involved in bridging the DSPP mutations and the resulting abnormal dentine mineralisation. The DSPP encoding proteins DSP (dentine sialoprotein) and DPP (dentine phosphoprotein) are positive regulators of dentine formation and perform a function during dentinogenesis.

View Article and Find Full Text PDF

Chemical cleaning and disinfection are crucial steps for eliminating infection in root canal treatment. However, irrigant selection or irrigation procedures are far from clear. The vapor lock effect in the apical region has yet to be solved, impeding irrigation efficacy and resulting in residual infections and compromised treatment outcomes.

View Article and Find Full Text PDF

Endodontic diseases are a kind of chronic infectious oral disease. Common endodontic treatment concepts are based on the removal of inflamed or necrotic pulp tissue and the replacement by gutta-percha. However, it is very essential for endodontic treatment to debride the root canal system and prevent the root canal system from bacterial reinfection after root canal therapy (RCT).

View Article and Find Full Text PDF
Article Synopsis
  • Human dental pulp stem cells (hDPSCs) exist in the dental pulp's specialized "niches," where sensory nerves help their growth and differentiation, while the role of sympathetic nerves is less understood.
  • The study explored how the sympathetic nervous system (SNS) affects hDPSCs by examining nerve fiber distribution, norepinephrine levels in dental pulp, and using various assays to assess hDPSCs' behavior.
  • Findings revealed that sympathetic nerve fibers and norepinephrine levels increased in inflamed pulps, with the major neurotransmitter receptor ADRA1B regulating hDPSCs' proliferation and migration via specific metabolic pathways.
View Article and Find Full Text PDF

Objectives: This study was aimed at evaluating the clinical and radiological outcomes of novel dynamic navigation (DN)-aided endodontic microsurgery (EMS), with an analysis of potential prognostic factors.

Materials And Methods: Forty-six teeth from 32 patients who received DN-aided EMS were included. Clinical and radiographic assessments were performed at least 1 year postoperatively.

View Article and Find Full Text PDF
Article Synopsis
  • - NSOFCs, the most prevalent craniofacial birth defects, include types such as nonsyndromic cleft lip with or without cleft palate and cleft palate only, with risk factors only partially explaining their heritability.
  • - Researchers conducted a comprehensive GWAS involving a large sample size from the Chinese Han population, identifying 47 genetic risk loci associated with NSOFCs, including 5 new loci.
  • - The identified loci account for about 44.12% of the heritability of NSOFCs, shedding light on the genetic factors influencing craniofacial anomalies.
View Article and Find Full Text PDF

Objectives: To evaluate the accuracy of a dynamic navigation system (DNS) for guided osteotomy and root-end resection during endodontic microsurgery (EMS) and assess its prognosis.

Methods: Nine patients who met inclusion criteria underwent DNS-guided EMS. Osteotomy and root-end resection were performed with assistance of DNS (DHC-ENDO1, DCARER Medical Technology, Suzhou, China).

View Article and Find Full Text PDF

Aim: Pyroptosis is a type of inflammatory cell death and is related to pulpitis and apical periodontitis. In this study, the aim was to investigate how periodontal ligament fibroblasts (PDLFs) and dental pulp cells (DPCs) respond to pyroptotic stimuli and explore whether dimethyl fumarate (DMF) could block pyroptosis in PDLFs and DPCs.

Methodology: Three methods (stimulation with lipopolysaccharide [LPS] plus nigericin, poly(dA:dT) transfection and LPS transfection) were used to induce pyroptosis in PDLFs and DPCs, two types of fibroblasts related to pulpitis and apical periodontitis.

View Article and Find Full Text PDF

Background: Tooth agenesis (TA) is a congenital abnormality that may present as syndromic or nonsyndromic. Considering its complex genetic aetiology, the aim of this study was to uncover the pathogenic mutants in patients with nonsyndromic TA and analyse the characteristics of these mutants.

Methods: Exome sequencing was performed to detect pathogenic variants in 72 patients from 43 unrelated families with nonsyndromic TA.

View Article and Find Full Text PDF

Background: Epidemiological studies have identified the role of periodontitis in the pathogenesis of type 2 diabetes, but the underlying mechanism is poorly understood. It is well-known that small extracellular vesicles are lipid bilayer vesicles derived from cells with a diameter around 30 to 200 nm. The purpose of this study was to investigate whether periodontitis induced or exacerbated insulin resistance via circulating small extracellular vesicles.

View Article and Find Full Text PDF

When nonsurgical endodontic treatment fails, surgical treatment is an alternative approach for treating periapical disease. However, endodontic microsurgery (EMS), particularly in anatomically challenging areas, such as the posterior teeth, is a skill-sensitive task that can present a unique set of challenges for the surgeon. In recent years, digital guidance technology has been applied more frequently in dentistry.

View Article and Find Full Text PDF

Introduction: Odontoblasts, terminally differentiated dentin-forming cells with their processes that penetrate into dentin, have been considered potential sensory cells. Current research suggests that odontoblasts sense external stimuli and transmit pain signals. PIEZO1, as a specific mechanically activated ion channel, may play an important role in mechanical transduction in odontoblasts.

View Article and Find Full Text PDF

Background: Commitment of mouse dental papilla cells (mDPCs) to the odontoblast lineage is critical for dentin formation, and this biological process is regulated by a complex transcription factor network. The transcription factor Mycn is a proto-oncogene that plays an important role in tumorigenesis and normal embryonic development. An early study revealed that Mycn is exclusively expressed in dental mesenchymal cells at E15.

View Article and Find Full Text PDF

Nonsyndromic orofacial clefts (NSOFCs) are the most common congenital defects in the oral and maxillofacial regions. It is mainly diagnosed prenatally through fetal ultrasonography. However, the accuracy of ultrasonography for NSOFC is unreliable.

View Article and Find Full Text PDF

The cellular atlas of the stroma is not well understood. Here, the cell populations in human dental pulp through single-cell RNA sequencing are profiled. Dental pulp stem cells, pulp cells, T cells, macrophages, endothelial cells, and glial cells are identified in human dental pulp.

View Article and Find Full Text PDF

Non-syndromic cleft lip with or without cleft palate (NSCL/P) is the most common subphenotype of non-syndromic orofacial clefts arising from genetic and/or environmental perturbations during embryonic development. We previously identified 2p24.2 as a risk locus associated with NSCL/P in the Chinese Han population, and MYCN is a candidate risk gene in this region.

View Article and Find Full Text PDF

Sulforaphane (SFN), an isothiocyanate naturally occurring in cruciferous vegetables, is a potent indirect antioxidant and a promising agent for the control of metabolic disorder disease. The glucose intolerance and adipogenesis induced by diet in rats was inhibited by SFN. Strategies aimed at induction of brown adipose tissue (BAT) could be a potentially useful way to against obesity.

View Article and Find Full Text PDF

Background And Objective: Activated potassium channels were found to be strongly correlated with gingival overgrowth (GO) phenotype as we reviewed syndromic hereditary gingival fibromatosis (HGF). Nevertheless, the functional roles of potassium channels in gingival fibrosis or gingival overgrowth remained uncovered. The aim of the present study was to explore the pathogenic role of aberrantly activated potassium channel in Hereditary Gingival Fibromatosis (HGF).

View Article and Find Full Text PDF

A PHP Error was encountered

Severity: Warning

Message: fopen(/var/lib/php/sessions/ci_sessiondn2cq7s2dsopusfrsd76aa5dbgc7nred): Failed to open stream: No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 177

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once

A PHP Error was encountered

Severity: Warning

Message: session_start(): Failed to read session data: user (path: /var/lib/php/sessions)

Filename: Session/Session.php

Line Number: 137

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once