Gas-phase fluorescence excitation and emission spectroscopy of mass-selected trapped molecular ions.

A flexible interface to perform optical spectroscopic measurements on gaseous ions stored in a modified commercial quadrupole ion trap (QIT) mass spectrometer is described. The modifications made to the mass spectrometer did not adversely affect its operating characteristics. Gas-phase ions are produced using electrospray ionization, mass isolated and stored in the trapping mass spectrometer.

[Effects of Qishe Pill on vertebral hyperostosis induced by upright posture in rats].

Objective: To observe the effects of Qishe Pill, a compound traditional Chinese herbal medicine, on lumbar vertebral bone formation induced by long-time upright posture in rats and to investigate the potential mechanism.

Methods: Thirty SD rats were randomly divided into normal control group, untreated group and Qishe Pill group. The rats in normal control group received no treatment and were raised in normal cages.

Bis[1-(3,5-di-tert-butyl-2-hydroxy-benz-yl)-3-isopropyl-imidazolium] penta-chlorido(tetra-hydro-furan)samarate(III)-tetrahydrofuran-toluene (1/1/1).

The title compound, (C(21)H(33)N(2)O)(2)[SmCl(5)(C(4)H(8)O)]·C(7)H(8)·C(4)H(8)O, has a layered structure in which each distorted octa-hedral [SmCl(5)(THF)](2-) unit (THF is tetra-hydro-furan) is capped by two cations. The central metal Sm(III) atom of the [SmCl(5)(THF)](2-) anionic unit is coordinated by five Cl atoms and one THF O atom, forming a distorted octa-hedral geometry. The crystal structure displays C-H⋯Cl and O-H⋯Cl hydrogen bonding.

Proteasome inactivation promotes p38 mitogen-activated protein kinase-dependent phosphatidylinositol 3-kinase activation and increases interleukin-8 production in retinal pigment epithelial cells.

Oxidative stress and inflammation are implicated in the pathogenesis of many age-related diseases. We have demonstrated previously that oxidative inactivation of the proteasome is a molecular link between oxidative stress and overexpression of interleukin (IL)-8. Here, we elucidated a novel signaling cascade that leads to up-regulation of IL-8 in response to proteasome inactivation.

Highly enantioselective zinc/amino alcohol-catalyzed alkynylation of aldehydes.

Meeting the challenge: The zinc/amino alcohol catalyzed enantioselective addition of terminal alkynes to aldehydes is effective with both phenylacetylene and methyl propiolate, leading to chiral secondary propargyl alcohols with very high enantioselectivity (see scheme).

[Preventive and therapeutic effects of Yiqi Huayu Recipe on degeneration of articular cartilage in rats with osteoarthritis].

Objective: To study the efficacy and possible mechanism of Yiqi Huayu Recipe (YQHYR), a compound traditional Chinese herbal medicine, in preventing and treating degeneration of the articular cartilage in rats with osteoarthritis (OA).

Methods: A total of 90 one-month-old SD rats were randomly divided into normal control group, untreated group and YQHYR group, with 30 rats in each group. The osteoarthritis was induced by shoulder disarticulation plus upright posture in rats.

[Mechanism of Yiqi Huayu Bushen recipe in treating cervical syndrome in rats with qi deficiency, blood stasis and kidney deficiency].

Objective: To explore the mechanism of Yiqi Huayu Bushen Recipe (YHBR), a compound traditional Chinese herbal medicine, in treating cervical syndrome (CS) with qi deficiency, blood stasis and kidney deficiency in rats.

Methods: A total of 30 three-month-old female Sprague-Dawley rats were randomly divided into normal control group, untreated group and YHBR group; there were ten rats in each group. The rat model of cervical syndrome with qi deficiency, blood stasis and kidney deficiency was established by combining disease and syndrome models.

[Mechanism of Yiqi Huayu Bushen Recipe in treating cervical syndrome with kidney deficiency in rats].

Objective: To research the action mechanism of Yiqi Huayu Bushen Recipe (YHBR), a compound of traditional Chinese herbal medicine, in treating cervical syndrome (CS) with kidney deficiency in rats.

Methods: A total of 30 three-month-old female Sprague-Dawley rats were randomly divided into normal control group, CS with kidney deficiency model group (untreated group) and YHBR group, with 10 rats in each group. Rats in the normal control group received no treatment, while rats of the other two groups underwent resection of both cervical muscles and ovaries to establish the model of CS with kidney deficiency.

[Establishment of a rat model of cervical syndrome with qi deficiency, blood stasis and kidney deficiency].

Objective: To establish a rat model of cervical syndrome with qi deficiency, blood stasis and kidney deficiency.

Methods: A total of 30 three-month-old female Sprague-Dawley rats were randomly divided into normal group, cervical syndrome group and cervical syndrome with qi deficiency, blood stasis and kidney deficiency group (combined group), with 10 rats in each group. Rats in the normal group received no treatment, rats in cervical syndrome group underwent resection of cervical muscles and ligaments as unbalanced dynamic and static animal model, and rats in combined group underwent resection of both cervical muscles and ovaries, swimming and irregular diet, and injection of adrenal cortex hormone and adrenaline two and a half months after resection as combined model.

Sustained oxidative stress inhibits NF-kappaB activation partially via inactivating the proteasome.

NF-kappaB is a family of important transcription factors involved in many cellular functions, such as cell survival, proliferation, and stress responses. Many studies indicate that NF-kappaB is a stress-sensitive transcription factor and its activation is regulated by reactive oxygen species. In previous studies, we and others demonstrated that this transcription factor can be activated by transient oxidative stress.

[Establishment of a rat model of cervical syndrome with kidney deficiency].

Objective: To establish a rat model of cervical syndrome (CS) with kidney deficiency.

Methods: A group of 30 three-month-old female Sprague-Dawley rats were randomly divided into normal control group, CS group and CS with kidney deficiency group (combined group), with 10 rats in each group. Rats in the normal control group received no treatment, rats in the CS group underwent only resection of cervical muscles and ligaments as unbalanced dynamic and static animal model, and rats in combined group underwent resection of both cervical muscles and ovaries as kidney deficiency model.

Oxidative inactivation of the proteasome in retinal pigment epithelial cells. A potential link between oxidative stress and up-regulation of interleukin-8.

Oxidative stress and inflammation are implicated in the pathogenesis of many age-related diseases. Stress-induced overproduction of inflammatory cytokines, such as interleukin-8 (IL-8), is one of the early events of inflammation. The objective of this study was to elucidate mechanistic links between oxidative stress and overproduction of IL-8 in retinal pigment epithelial (RPE) cells.

Expression of K6W-ubiquitin in lens epithelial cells leads to upregulation of a broad spectrum of molecular chaperones.

Purpose: Accumulation and precipitation of abnormal proteins are associated with many age-related diseases. The ubiquitin-proteasome pathway (UPP) is one of the protein quality control mechanisms that selectively degrade damaged or obsolete proteins. The other arm of the protein quality control mechanism is molecular chaperones, which bind to and help refold unfolded or misfolded proteins.

Lithium reduces ischemia-induced hippocampal CA1 damage and behavioral deficits in gerbils.

Lithium is a major drug used for the treatment of bipolar mood disorder and has recently been shown to have neuroprotective properties. In this study we investigated the neuroprotective effects of lithium in gerbils subjected to global cerebral ischemia, an animal model of stroke. The ischemia-induced exploratory behavior changes, measured by open field testing, were largely suppressed by lithium treatment for 7 days prior to ischemic onset.

The effect of atypical antipsychotics, perospirone, ziprasidone and quetiapine on microglial activation induced by interferon-gamma.

An accumulating body of evidences point to the significance of neuroinflammation and immunogenetics in schizophrenia, characterized by increased serum concentration of several pro-inflammatory cytokines. In the central nervous system (CNS), the microglial cells are the major immunocompetent cells which release pro-inflammatory cytokines, nitric oxide (NO) and reactive oxygen species to mediate the inflammatory process. In the present study, we investigated whether or not atypical antipsychotics, namely perospirone, quetiapine and ziprasidone, would have anti-inflammatory effects on the activated microglia which may potentiate neuroprotection.

[Experimental investigation of CAEP far-infra-red 100 microm FEL spontaneous emission].

In the FEL experiment, when electron beam passes through the wiggler, the movement of electron beam will be changed to wiggle in the wiggler due to the periodical magnet field. Meanwhile the electron beam will spontaneously emit the radiation with a wavelength similiar to the FEL radiation. This radiation is influenced by the parameters of both the electron beam and the wiggler.

Octylphenol induces apoptosis in cultured rat Sertoli cells.

In this study, the effects of 4-tert-octylphenol (OP) were examined on the viability of rat cultured Sertoli cells using the MTT assay and OP-induced apoptosis was detected by transmission electron microscope (TEM), flow cytometric analysis and Hoechst staining. In addition, RT-PCR was used to analyze the levels of Bcl-2 and Bax mRNA. Bcl-2, Bax and caspase-3 protein expressions were determined by Western blot analysis.

[Analysis of the found age and way for children age 0 to 6 with hearing loss].

Objective: To investigate the children with hearing loss from the age 0 to 6, and discuss the found age, found way and audiological characteristics.

Methods: General information of found age and found way of 265 children, were investigated with self-made questionnaire and routine audiological evaluations, and then made statistical analysis.

Results: The average (x +/- s) found age for the children with hearing loss was (23.

[Research on the interaction of aristolochic acid and bovine serum albumin].

The interaction between aristolochic acid and bovine serum albumin (BSA) under physiological conditions was investigated by fluorescence quenching methods. The results indicate that there is a strong interaction between aristolochic acid and BSA, and the distances between the binding location and tryptophan residue is 2.8 nm.

Cervical intervertebral disc degeneration induced by unbalanced dynamic and static forces: a novel in vivo rat model.

Study Design: Establishment of a novel in vivo animal model of cervical spondylosis.

Objective: To investigate apoptotic, degenerative, and inflammatory changes occurring in the cervical intervertebral discs of rats.

Summary Of Background Data: Cervical degeneration occurs as the result of imbalance of both static and dynamic spinal stabilizers.

[Clinical and pathological characteristics in patients with clinically presumed hypertensive nephrosclerosis].

Objective: To investigate the clinical and pathological characteristics of patients with clinically presumed hypertensive nephrosclerosis (HN).

Methods: Clinical data and renal biopsy results were obtained in 63 patients diagnosed clinically as HN (primary hypertension plus renal injury).

Results: HN was confirmed by biopsy in 47 out of 63 patients (74.

[An in vitro natural degeneration model of chondrocytes derived from endplate of intervertebral discs of rats].

Objective: To set up a natural degeneration model of chondrocytes derived from endplate of intervertebral discs of rats in order to offer an appropriate carrier for the study on mechanism of intervertebral disc degeneration.

Methods: The method of enzyme digestion combined with natural subculture was used to set up the in vitro natural degeneration model of chondrocytes derived from the endplate of intervertebral disc of rats. The morphological appearances and microstructures of the chondrocytes of different generations were observed.

The toxic effects of 4-tert-octylphenol on the reproductive system of male rats.

The aim of the present study is to investigate the toxic effect of 4-tert-octylphenol (OP) on testicular functions of rats. Male Sprague-Dawley rats were orally administered different doses of OP at the levels of 0 (control), 50, 150 and 450 mg/kg/d for 30 d. Testicular functions were assessed by histopathology, testicular sperm head counts, daily sperm production, sperm motility (measured by computer assisted sperm analysis, CASA) and biochemical indices (marker testicular enzymes).

[Simultaneous determination of the four effective components in Huaijiao pill by HPLC].

Objective: To develop an HPLC method for determination of the four effective components (genistin; rutin; quercetin; genistein) in Huaijiao pill.

Method: The chromatographic separation was performed on a Shim-packODS (4.6 mm x 150 mm, 5.