Metabolic engineering of for high-level production of pneumocandin B.

Pneumocandin B (PB) is a lipohexapeptide synthesized by and serves as the precursor for the widely used antifungal drug caspofungin acetate (Cancidas®). However, the low titer of PB results in fermentation and purification costs during caspofungin production, limiting its widespread clinical application. Here, we engineered an efficient PB-producing strain of by systems metabolic engineering strategies, including multi-omics analysis and multilevel metabolic engineering.

Coherent solvus of disordered alkali feldspar: experiment, atom probe tomography and thermodynamic model.

Unlabelled: At temperatures above about 600 °C, alkali feldspar forms a continuous solid solution between the Na and K end members. Towards lower temperatures a miscibility gap opens, and alkali feldspar of intermediate composition exsolves, forming an intergrowth of relatively more Na-rich and K-rich lamellae. During exsolution, the crystal structure usually remains coherent across the lamellar interfaces, a feature that may be preserved over geological times.

Establishing a nomogram for predicting the risk factors for delayed bleeding after endoscopic submucosal dissection for colorectal tumors.

Background: The aim of this study is to establish a nomogram that can predict the risk factors for delayed bleeding after endoscopic submucosal dissection (ESD). This model can be used to assess the probability of delayed bleeding before ESD surgery, thereby avoiding wasting medical resources and improving patient satisfaction.

Methods: This was a retrospective study in which all patients underwent ESD surgery for colorectal tumors between August 2021 and February 2024.

Corrigendum: Case report: A golden tail of immunotherapy: significant tail effect in a chemotherapy-resistant advanced pulmonary sarcomatoid carcinoma patient treated by Sintilimab combined with Anlotinib.

[This corrects the article DOI: 10.3389/fimmu.2024.

Chemical Synthesis of ~1 nm Multilevel Capacitor-like Particles with Atomic Precision.

Can the chemically synthesized nanoparticles act as nanodevices or nanomachines? Herein, we demonstrated this feasibility. A novel nanocluster (ultrasmall nanoparticle) [AuCd(m-MBT)][N(CH)] (AuCd in short, m-MBTH: m-methylbenzenethiol) obtained via developing a synthesis method has a cannula-like structure of the outer shell and an internal sleeve, revealed by single-crystal X-ray diffraction. Natural population analysis (NPA) charge calculations, charge carrier transport of AuCd (during which an intra-nanocluster anti-galvanic reaction was observed) after unneutral charging using NaBH as well as voltammetry proved the capacitor-like character of AuCd.

Evidence of Directional Structural Superlubricity and Lévy Flights in a van der Waals Heterostructure.

Structural superlubricity is a special frictionless contact in which two crystals are in incommensurate arrangement such that relative in-plane translation is associated with vanishing energy barrier crossing. So far, it has been realized in multilayer graphene and other van der Waals (2D crystals with hexagonal or triangular crystalline symmetries, leading to isotropic frictionless contacts. Directional structural superlubricity, to date unrealized in 2D systems, is possible when the reciprocal lattices of the two crystals coincide in one direction only.

Exosome applications for the diagnosis and treatment of pancreatic ductal adenocarcinoma: An update (Review).

Pancreatic ductal adenocarcinoma (PDAC) is a malignant neoplasm that typically manifests with subtle clinical manifestations in its early stages and frequently eludes diagnosis until the advanced phases of the disease. The limited therapeutic options available for PDAC significantly contribute to its high mortality rate, highlighting the urgent need for novel biomarkers capable of effectively identifying early clinical manifestations and facilitating precise diagnosis. The pivotal role of cellular exosomes in both the pathogenesis and therapeutic interventions for PDAC has been underscored.

Case report: A golden tail of immunotherapy: significant tail effect in a chemotherapy-resistant advanced pulmonary sarcomatoid carcinoma patient treated by Sintilimab combined with Anlotinib.

Tail effect is a unique phenomenon in immunotherapy characterized by the prolonged maintenance of therapeutic efficacy. It can be observable even after treatment cessation. Immunotherapy has gradually become a vital regimen for the treatment of advanced lung cancer patients, among which immune-combined therapies based on immune checkpoint inhibitors (ICIs) have been applied clinically and demonstrates considerable clinical efficacy.

Substantially Enhanced Spin Polarization in Epitaxial CrTe Quantum Films.

2D van der Waals (vdW) magnets, which extend to the monolayer (ML) limit, are rapidly gaining prominence in logic applications for low-power electronics. To improve the performance of spintronic devices, such as vdW magnetic tunnel junctions, a large effective spin polarization of valence electrons is highly desired. Despite its considerable significance, direct probe of spin polarization in these 2D magnets has not been extensively explored.

A comparative study of the effects of urban morphology on land surface temperature in Chengdu and Chongqing, China.

Urbanization combined with global climate change, exacerbates the urban thermal environment and hinders sustainable urban development. However, the complex relationships between land surface temperature (LST) and urban morphology are being further understood, particularly in relation to different urban development patterns, distinct topography, and 3D building morphology. Thus, this study conducted a comparative study in Chengdu and Chongqing, Southwest China.

Trends and Prospects of Low-Density Lipoprotein Cholesterol in Stroke: A Bibliometric Analysis.

Management of low-density lipoprotein cholesterol (LDL-C) in stroke is a crucial component of cardiovascular disease care. Recent years have seen substantial progress in understanding and managing LDL-C in the context of stroke. This study utilized bibliometric methods to analyze and synthesize trends in this area over the past decade, incorporating 2,841 publications from the Web of Science database.

Exposure to PFOA, PFOS, and PFHxS induces Alzheimer's disease-like neuropathology in cerebral organoids.

Per- and polyfluoroalkyl substances (PFASs), a class of ubiquitous synthetic organic chemicals, are widely utilized across various industrial applications. However, the long-term neurological health effects of PFAS mixture exposure in humans remain poorly understood. To address this gap, we have designed a comprehensive study to predict and validate cell-type-specific neurotoxicity of PFASs using single-cell RNA sequencing (scRNA-seq) and cerebral organoids.

Method for Augmenting Side-Scan Sonar Seafloor Sediment Image Dataset Based on BCEL1-CBAM-INGAN.

In this paper, a method for augmenting samples of side-scan sonar seafloor sediment images based on CBAM-BCEL1-INGAN is proposed, aiming to address the difficulties in acquiring and labeling datasets, as well as the insufficient diversity and quantity of data samples. Firstly, a Convolutional Block Attention Module (CBAM) is integrated into the residual blocks of the INGAN generator to enhance the learning of specific attributes and improve the quality of the generated images. Secondly, a BCEL1 loss function (combining binary cross-entropy and L1 loss functions) is introduced into the discriminator, enabling it to focus on both global image consistency and finer distinctions for better generation results.

Hesperidin Helps Improve the Intestinal Structure, Maintain Barrier Function, and Reduce Inflammation in Yellow-Feathered Broilers Exposed to High Temperatures.

To investigate the possible protective effect of hesperidin on intestinal damage caused by high-temperature heat stress in yellow-feathered broilers, 960 broilers aged 21 days were randomly divided into four groups: HT, HT300, HT450, and HT600, with each group receiving different amounts of hesperidin supplementation (0, 300, 450, and 600 mg/kg). The dietary supplementation of hesperidin could mitigate the elevation of corticosterone (CORT) and adrenocorticotropic hormone (ATCH) levels in serum from yellow-feathered broilers induced by heat stress. The supplementation of 300 mg/kg and 450 mg/kg of hesperidin reduced crypt depth and increased the V/C ratio in the small intestine compared to the HT group.

Few-Human-Interaction Reinforcement Learning for Autonomous Transbronchial Intervention.

The transbronchial interventional surgery presents challenges with winding and convoluted pathways, prone to compression and friction. Current autonomous planning struggles to reach deeper bronchial positions, and hard to consider multiple conflicting goals simultaneously. This article introduces an innovative planning scheme with preference weights to achieve smooth, frictionless, and collision-free autonomous transbronchial intervention with continuum robot (CR).

Gapped and Rotated Grain Boundary Revealed in Ultra-Small Au Nanoparticles for Enhancing Electrochemical CO Reduction.

Although gapped grain boundaries have often been observed in bulk and nanosized materials, and their crucial roles in some physical and chemical processes have been confirmed, their acquisition at ultrasmall nanoscale presents a significant challenge. To date, they had not been reported in metal nanoparticles smaller than 2 nm owing to the difficulty in characterization and the high instability of grain boundary (GB) atoms. Herein, we have successfully developed a synthesis method for producing a novel chiral nanocluster Au(TBBT) (TBBT=4-tert-butylphenylthiolate) with a 26-atom gapped and rotated GB.

The associations of social isolation with depression and anxiety among adults aged 65 years and older in Ningbo, China.

Social isolation was associated with emotional problems (depression and anxiety) among older adults, however, little is known in China. Thus, we conducted a cross-sectional study including 6,664 ≥ 65 years older adults in Ningbo, China. We collected data on social isolation, depression, and anxiety by specific scales.

DS-SIAUG: A Self-Training Approach Using a Disrupted Student Model for Enhanced Side-Scan Sonar Image Augmentation.

Side-scan sonar is a principal technique for subsea target detection, where the quantity of sonar images of seabed targets significantly influences the accuracy of intelligent target recognition. To expand the number of representative side-scan sonar target image samples, a novel augmentation method employing self-training with a Disrupted Student model is designed (DS-SIAUG). The process begins by inputting a dataset of side-scan sonar target images, followed by augmenting the samples through an adversarial network consisting of the DDPM (Denoising Diffusion Probabilistic Model) and the YOLO (You Only Look Once) detection model.

Multi-Scale Marine Object Detection in Side-Scan Sonar Images Based on BES-YOLO.

Aiming at the problem of low accuracy of multi-scale seafloor target detection in side-scan sonar images with high noise and complex background texture, a model for multi-scale target detection using the BES-YOLO network is proposed. First, an efficient multi-scale attention (EMA) mechanism is used in the backbone of the YOLOv8 network, and a bi-directional feature pyramid network (Bifpn) is introduced to merge the information of different scales, finally, a Shape_IoU loss function is introduced to continuously optimize the model and improve its accuracy. Before training, the dataset is preprocessed using 2D discrete wavelet decomposition and reconstruction to enhance the robustness of the network.

Realization of a two-dimensional Weyl semimetal and topological Fermi strings.

A two-dimensional (2D) Weyl semimetal, akin to a spinful variant of graphene, represents a topological matter characterized by Weyl fermion-like quasiparticles in low dimensions. The spinful linear band structure in two dimensions gives rise to distinctive topological properties, accompanied by the emergence of Fermi string edge states. We report the experimental realization of a 2D Weyl semimetal, bismuthene monolayer grown on SnS(Se) substrates.

Conversion of Propargylic Amines with CO Catalyzed by a Highly Stable Copper(I) Iodide Thorium-Based Heterometal-Organic Framework.

The conversion of CO to generate high-value-added chemicals has become one of the hot research topics in green synthesis. Thereinto, the cyclization reaction of propargylic amines with CO is highly attractive because the resultant oxazolidinones are widely found in pharmaceutical chemistry. Cu(I)-based metal-organic frameworks (MOFs) as catalysts exhibit promising application prospects for CO conversion.