Publications by authors named "Bialek R"

In the paper, a new design of a quasi-isotropic antenna for high-power electromagnetic (EM) field measurement is presented, along with its investigation into suitability. The measuring probe is intended for assessing pulsed microwaves, which cannot be measured by available meters due to the high value of electric field strength and short pulse duration. The measurement of such a strong field is required according to guidelines for protecting people against microwave fields, especially those emitted by radars.

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Background: Among combat injured, invasive fungal infections (IFIs) result in significant morbidity. Cultures and histopathology are the primary diagnostic methods for IFIs, but they have limitations. We previously evaluated a panfungal polymerase chain reaction assay, which was 83% sensitive and 99% specific for angioinvasive IFIs.

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Context: Health departments nationally are critically understaffed and lack infrastructure support. By examining current staffing and allocations through a Foundational Public Health Services (FPHS) lens at the Northern Nevada Public Health (NNPH), there is an opportunity to make a strong case for greater investment if current dedicated full-time equivalents are inadequate and to guide which investments in public health workforce are prioritized.

Objective: To assess the use of the Public Health Workforce Calculator (calculator) and other tools to identify and prioritize FPHS workforce needs in a field application.

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Observation of Fano resonances in various physical phenomena is usually ascribed to the coupling of discrete states with background continuum, as it has already been reported for various physical phenomena. Here, we report on Fano lineshapes of nonthermal GHz phonons generated and observed with pumped Brillouin light scattering in gold-silicon thin membranes, overlapping the broad zero-shift background of yet questionable origin. The system's broken mid-plane symmetry enabled the generation of coherent quasi-symmetric and quasi-antisymmetric Lamb acoustic waves/phonons, leading to the four orders-of-magnitude enhancement of Brillouin light scattering.

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This study presents the outcome of research into membrane vibrations in a 3D space performed using a system based on position sensitive device (PSD) sensors. Here, measurements were conducted for harmonic vibrations. The use of such detectors for assessing the movement of objects within a plane or space requires determining the position of more than one marker.

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In recent years, many public health organizations have used 2 frameworks for workforce development planning and action: the Core Competencies for Public Health Professionals (Core Competencies) and the Strategic Skills for the Governmental Public Health Workforce (Strategic Skills). A third framework is also available for organizations emphasizing population health: the Competencies for Population Health Professionals (Population Health Competencies). This crosswalk analysis-conducted by the Public Health Foundation with input from the Region 2 Public Health Training Center and the de Beaumont Foundation-harmonizes these 3 schemas by systematically mapping the 2014 version of the Core Competencies and the 2019 Population Health Competencies with the 2017 version of the Strategic Skills to produce a comprehensive matrix depicting their relationships.

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Paracoccidioidomycosis (PCM) is a systemic mycosis caused by a group of cryptic species embedded in the Paracoccidioides brasiliensis complex and Paracoccidioides lutzii. Four species were recently inferred to belong to the P. brasiliensis complex, but the high genetic diversity found in both human and environmental samples have suggested that the number of lineages may be higher.

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Light-induced electron-transfer reactions were investigated in wild-type and three mutant reaction centers with the secondary electron acceptor (ubiquinone Q) either removed or permanently reduced. Under such conditions, charge separation between the primary electron donor (bacteriochlorophyll dimer, P) and the electron acceptor (bacteriopheophytin, H) was followed by PH → PH charge recombination. Two reaction centers were used that had different single amino-acid mutations that brought about either a 3-fold acceleration in charge recombination compared to that in the wild-type protein, or a 3-fold deceleration.

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We investigated the influence of a range of factors-temperature, redox midpoint potential of an electron carrier, and protein dynamics-on nanosecond electron transfer within a protein. The model reaction was back electron transfer from a bacteriopheophytin anion, H, to an oxidized primary electron donor, P, in a wild type Rhodobacter sphaeroides reaction center (RC) with a permanently reduced secondary electron acceptor (quinone, Q). Also used were two modified RCs with single amino acid mutations near the monomeric bacteriochlorophyll, B, located between P and H.

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Article Synopsis
  • Colloidal quantum dots (QDs) can both photoreduce redox proteins through electron transfer (ET) and transfer energy via resonance energy transfer (RET), prompting an investigation of how these processes compete in quenching QD excitation.
  • The study involved various forms of cytochrome c (CytC), which demonstrated that all tested derivatives could quench QD fluorescence through different quenching mechanisms, with the effectiveness influenced by QD size.
  • Results indicated a preference for photoinduced ET over RET, partly due to the formation of stable protein-QD complexes, and suggest that RET might also contribute to enhancing ET processes.
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The EORTC/MSGERC have revised the definitions for proven, probable, and possible fungal diseases. The tissue diagnosis subcommittee was tasked with determining how and when species can be determined from tissue in the absence of culture. The subcommittee reached a consensus decision that polymerase chain reaction (PCR) from tissue, but not immunohistochemistry or in situ hybridization, can be used for genus or species determination under the new EORTC/MSGERC guidelines, but only when fungal elements are identified by histology.

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Biohybrid photoelectrochemical systems in photovoltaic or biosensor applications have gained considerable attention in recent years. While the photoactive proteins engaged in such systems usually maintain an internal charge separation quantum yield of nearly 100%, the subsequent steps of electron and hole transfer beyond the protein often limit the overall system efficiency and their kinetics remain largely uncharacterized. To reveal the dynamics of one of such charge-transfer reactions, we report on the reduction of reaction centers (RCs) by Os-complex-modified redox polymers (P-Os) characterized using transient absorption spectroscopy.

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Excitation decay in closed Photosystem I (PSI) isolated from cyanobacterium Synechocystis sp. PCC 6803 and dissolved in a buffer solution occurs predominantly with a ~ 24-ps lifetime, as measured both by time-resolved fluorescence and transient absorption. The same PSI particles deposited in mesoporous matrix made of TiO nanoparticles exhibit significantly accelerated excitation decay dominated by a ~ 6-ps component.

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We demonstrate photovoltaic activity of electrodes composed of fluorine-doped tin oxide (FTO) conducting glass and a multilayer of trimeric photosystem I (PSI) from cyanobacterium Synechocystis sp. PCC 6803 yielding, at open circuit potential (OCP) of + 100 mV (vs. SHE), internal quantum efficiency of (0.

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Article Synopsis
  • Researchers isolated a supercomplex called PSI-LHCI from the extremophile alga Cyanidioschyzon merolae to study chlorophylls that are energetically uncoupled from the PSI reaction center, which could impact the efficiency of PSI-based solar cells.
  • By stirring the samples at a warm temperature, the amount of uncoupled chlorophylls increased significantly from less than 2% to 47% over 3.5 hours.
  • The study revealed that the uncoupled chlorophylls emitted light at a shorter wavelength (around 680 nm), suggesting that mechanical stirring causes a loss of red-emitting chlorophylls and alters the physical organization of the light-harvesting proteins attached to the PSI
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Background: Invasive fungal diseases (IFDs) remain important causes of morbidity and mortality. The consensus definitions of the Infectious Diseases Group of the European Organization for Research and Treatment of Cancer and the Mycoses Study Group have been of immense value to researchers who conduct clinical trials of antifungals, assess diagnostic tests, and undertake epidemiologic studies. However, their utility has not extended beyond patients with cancer or recipients of stem cell or solid organ transplants.

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Photosynthetic PSI-LHCI complexes from an extremophilic red alga C. merolae grown under varying light regimes are characterized by decreasing size of LHCI antenna with increasing illumination intensity [1]. In this study we applied time-resolved fluorescence spectroscopy to characterize the kinetics of energy transfer processes in three types of PSI-LHCI supercomplexes isolated from the low (LL), medium (ML) and extreme high light (EHL) conditions.

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Article Synopsis
  • Trauma-related invasive fungal wound infections (IFIs) can lead to serious health complications, making early detection and treatment crucial.
  • A study evaluated a PCR-based test for quickly identifying filamentous fungi in tissue samples from soldiers injured in Afghanistan, which showed high specificity (99%) but moderate sensitivity (63%).
  • The PCR method was notably better than traditional cultures in detecting certain fungi, particularly those from the Mucorales order, suggesting it could improve diagnosis speed and accuracy for fungal infections in trauma cases.
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Objective: The objectives of this study were (1) to obtain data on the current status of public health workforce training and the use of the Training Finder Real-Time Affiliate Network (TRAIN), a public health learning management platform, in state health departments, and (2) to use the data to identify organizational features that might be affecting training and to determine barriers to and opportunities for improving training.

Methods: We conducted structured interviews in 2014 with TRAIN administrators and performance improvement managers (n = 14) from 7 state health departments that were using TRAIN to determine training practices and barriers to training. We determined key organizational features of the 7 agencies, including training structure, required training, TRAIN administrators' employment status (full time or part time), barriers to the use and tracking of core competencies in TRAIN, training needs assessment methods, leadership support of training and staff development, and agency interest in applying for Public Health Accreditation Board accreditation.

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Scabies has been diagnosed surprisingly frequently in Germany in recent years, and the use of acaricides has risen markedly. Present figures indicate an increase in the prevalence/incidence of scabies, but do not prove or quantify it for the following reasons: (a) scabies is not a notifiable disease in Germany; (b) the diagnosis is not always confirmed lege artis by means of light microscopy or dermatoscopy (which may lead to a comparatively high proportion of false‐positive diagnoses due to the low overall prevalence of scabies); (c) repeated treatments of the same patient and treatment of contact persons are included in the total number of prescriptions. Therefore, there are no valid data on disease occurrence, either in the current situation or from previous periods.

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A 60-year-old man was admitted to the hospital with productive cough and yellowish sputum, severe fatigue, and weight loss of 4 kg over the past month; furthermore, he reported a slowly progressive shortness of breath on exertion over the past 6 months. Before admission, he received ampicillin/sulbactam (750 mg) orally twice daily for 7 days without significant clinical improvement.

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As one of a number of new technologies for the harnessing of solar energy, there is interest in the development of photoelectrochemical cells based on reaction centres (RCs) from photosynthetic organisms such as the bacterium Rhodobacter (Rba.) sphaeroides. The cell architecture explored in this report is similar to that of a dye-sensitized solar cell but with delivery of electrons to a mesoporous layer of TiO by natural pigment-protein complexes rather than an artificial dye.

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