Lactiplantibacillus plantarum JS19-assisted fermented goat milk alleviates d-galactose-induced aging by modulating oxidative stress and intestinal microbiota in mice.

Oxidative stress is a crucial factor in the age-related decline in physiological, genomic, metabolic, and immunological functions. We screened Lactiplantibacillus plantarum JS19, which has been shown to possess therapeutic properties in mice with ulcerative colitis. In this study, L.

Milk-derived antimicrobial peptides incorporated whey protein film as active coating to improve microbial stability of refrigerated soft cheese.

Nisin is the first FDA-approved antimicrobial peptide and shows significant antimicrobial activity against Gram-positive bacteria, but only a weakly inhibitory effect on Gram-negative bacteria. The aim of this study was to prepare whey protein-based edible films with the incorporation of milk-derived antimicrobial peptides (α-casein and α-casein) and compare their mechanical properties and potential application in cheese packaging with films containing nisin. These two antimicrobial peptides showed similar activity against B.

Untargeted metabolomics analysis of probiotic jujube juice and its anti-obesity effects on high-fat-diet-induced obese mice.

Background: Dietary intervention, including polyphenol consumption, is recognized as an effective strategy to prevent obesity. Although fermented jujube juice (FJJ) with lactic acid bacteria has been shown to be rich in polyphenols and have strong antioxidant properties, little is known about its anti-obesity properties.

Results: Untargeted metabolomics was employed to identify and analyze the differential metabolites between FJJ and raw jujube juice.

Evaluation of penicillin-resistance and probiotic traits in Lactobacillus plantarum during laboratory evolution.

The aim of this study was to obtain the Lactobacillus plantarum ATCC14917 with high-level resistance to penicillin and evaluate their probiotic traits using laboratory evolution assay and whole-genome sequencing. In penicillin environment, the minimum inhibitory concentration (MIC) of L. plantarum to penicillin increased from 1 μg/mL to 16 μg/mL and remained stable after the removal of antibiotic pressure, suggesting that the resistance acquisition to penicillin was an irreversible process.

Novel dual-recognition electrochemical biosensor for the sensitive detection of AFM in milk.

Aflatoxin M (AFM) is a highly carcinogenic and toxic compound that is commonly found in dairy foods such as milk. We developed an electrochemical MIP (molecularly imprinted polymer) and aptamer (Apt) based aptasensor (Apt-Au@PEIM/AFM/MIP-Apt/AuNPs)/GCE) with dual-recognition signal amplification to detect AFM in milk. The MIP-Apt/AuNP/GCE sensor was prepared by synthesizing an MIP film through the electropolymerization of resorcinol on gold nanoparticles-modified electrodes.

Rapid and label-free electrochemical aptasensor based on a palladium nanoparticles/titanium carbide/polyethyleneimine functionalized nitrogen-doped carbon nanotubes composite for amplified detection of streptomycin.

A novel electrochemical aptasensor for the determination of streptomycin (STR) was constructed in this work. We prepared a palladium nanoparticles/titanium carbide/polyethyleneimine-functionalized nitrogen-doped carbon nanotube (Pd@TiC-PEI-NCNTs) composite via hydrothermal-assisted formic acid reduction and employed it as a substrate material for anchoring a NH-functionalized aptamer (Apt) to the surface of an electrode through the Pd-N bond. The Pd@TiC-PEI-NCNTs composite increased the electrochemically active surface area and conductivity of the electrode, achieving substantial signal amplification (1.

Utilization of two plant polysaccharides to improve fresh goat milk cheese: Texture, rheological properties, and microstructure characterization.

This study aimed to evaluate the effects of added jujube polysaccharide (JP) and Lycium barbarum polysaccharide (LBP) on the texture, rheological properties, and microstructure of goat milk cheese. Seven groups of fresh goat milk cheese were produced with 4 levels (0, 0.2, 0.

Storage stability of texture, organoleptic, and biological properties of goat milk yogurt fermented with probiotic bacteria.

Introduction: Goat milk is an attractive food due to its high nutritional values, easy digestibility and hypoallergenicity, but has an undesirable "goaty" flavor.

Methods: In this study, goat yogurt was fermented with four probiotics, respectively, including (GYA), (GYB), (GYC) and (GYP), and tested for texture, organoleptic, and biological properties during a 4-week storage period at the refrigerated temperature.

Results: All goat yogurt with probiotics showed an increase on titratable acidity and a corresponding downward trend on pH value.

Therapeutic effect of Lactobacillus plantarum JS19 on mice with dextran sulfate sodium induced acute and chronic ulcerative colitis.

Background: Ulcerative colitis is associated with intestinal inflammation and dysbiosis. Previous studies have shown that probiotics are potential agents for treatment of inflammatory bowel disease (IBD). Jiang-shui is a traditional fermented vegetable that is rich in lactic acid bacteria (LABs), but the preventive effect of LABs in jiang-shui on IBD is not yet fully understood.

A label-free electrochemical aptasensor based on a gold nanoparticle/carbon nanotube/metal-organic framework nanohybrid for ultrasensitive detection of streptomycin in milk samples.

In this work, a novel electrochemical aptasensor was designed for the sensitive and specific detection of STR in milk samples. First, a gold nanoparticle@poly(diallyldimethylammonium chloride) nitrogen-doped carbon nanotube/polyethyleneimine-functionalized metal-organic framework (MOF) (Au@P-N-CNT/PEI-MIL-101(Cr)) composite was synthesized and characterized by various technique. The Au@P-N-CNT/PEI-MIL-101(Cr) composite was then modified on a bare glassy carbon electrode (GCE) surface, providing a favorable platform (Au@P-N-CNT/PEI-MIL-101(Cr)/GCE) for aptamer immobilization and current signal amplification.

An ultrasensitive sandwich-type electrochemical aptasensor using silver nanoparticle/titanium carbide nanocomposites for the determination of Staphylococcus aureus in milk.

A novel sandwich-type electrochemical aptasensor for the detection of Staphylococcus aureus (S. aureus) was developed. S.

A novel electrochemical aptasensor based on layer-by-layer assembly of DNA-Au@Ag conjugates for rapid detection of aflatoxin M in milk samples.

Aflatoxin M (AFM) is a common toxin in dairy products that causes acute and chronic human health disorders. Thus, the development of a rapid and accurate AFM detection method is of vital importance for food safety monitoring. This work was to develop a novel electrochemical aptasensor for sensitive and specific determination of AFM The dendritic-like nanostructure was formed on the gold electrode surface by layer-by-layer assembly of gold-silver core-shell nanoparticles modified with DNA conjugates.

Zinc in cereal grains: Concentration, distribution, speciation, bioavailability, and barriers to transport from roots to grains in wheat.

Zinc (Zn) is an essential micro-nutrient for humans, and Zn deficiency is of global concern. In addition to inherited and pathological Zn deficiencies, insufficient dietary intake is leading cause, especially in those consuming cereal grains as a stable food, in which Zn concentration and bioavailability are relatively low. To improve Zn levels in the human body, it is important to understand the accumulation and bioavailability of Zn in cereal grains.

Physicochemical and textural characteristics and volatile compounds of semihard goat cheese as affected by starter cultures.

Today, cheese is valued because of its high nutritional value and unique characteristics. Improving the texture and flavor of cheese by selecting suitable starter cultures is an important way to promote the development of cheese industry. The effect of starter cultures on the physicochemical and textural properties and volatile compounds during the ripening of semihard goat cheese were investigated in this work.

Quantitative Assessment of Phenolic Acids, Flavonoids and Antioxidant Activities of Sixteen Jujube Cultivars from China.

Increasing attention has been paid to the nutritional values and pharmacological effects of Ziziphus jujuba. Therefore, in this study, four phenolic and flavonoid fractions of 16 jujube cultivars from different geographic regions of China were separated and quantified. In addition, the antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH·) scavenging method and the ferric reducing antioxidant power (FRAP) assay.

Resveratrol and Oxyresveratrol Activate Thermogenesis via Different Transcriptional Coactivators in High-Fat Diet-Induced Obese Mice.

Obesity is a global public health issue. Thermogenesis is a novel way to promote anti-obesity by consuming energy as heat rather than storing it as triacylglycerols. The browning program allows mitochondrial biosynthesis and thermogenesis-related gene expression to occur in subcutaneous white adipose tissue, which results in the formation of beige adipose tissue.

Genome-wide analysis of fermentation and probiotic trait stability in Lactobacillus plantarum during continuous culture.

Trait stability of Lactobacillus plantarum was studied following daily subculture over a 90-d period. Acid and bile tolerance, self-aggregation ability, cell hydrophobicity, pathogen inhibition activity, and cholesterol removal ability of cultures subcultured 30 (Lp30), 60 (Lp60), or 90 (Lp90) times were not significantly different from the original strain (Lp0). However, carbohydrate metabolism patterns did change; the Lp0 culture was unable to use d-sorbitol, α-methyl-d-mannose, and d-raffinose, whereas Lp30, Lp60, and Lp90 cultures could.

Laboratory Evolution Assays and Whole-Genome Sequencing for the Development and Safety Evaluation of With Stable Resistance to Gentamicin.

The goal of this work was to use laboratory evolution assays and whole-genome sequencing to develop and test the safety of a probiotic, , with high-level of resistance to gentamicin. The evolution of was evaluated under the selective pressure from gentamicin and subsequently when the selective pressure was removed. After 30 days of selective pressure from gentamicin, the minimum inhibitory concentration (MIC) of to gentamicin increased from 4 to 512 μg/mL and remained stable at this level.

Optimized Extraction of Phenolics from Jujube Peel and Their Anti-inflammatory Effects in LPS-Stimulated Murine Macrophages.

The extraction of phenolics from jujube peel (PJP) was optimized using response surface methodology (RSM). A Box-Behnken design was utilized to analyze the effects of NaOH concentration, temperature, and extraction time on the total phenolic content (TPC). The results showed that RSM could be an adequate approach for modeling the extraction of PJP.

Quality characteristics and antioxidant activities of goat milk yogurt with added jujube pulp.

This study aimed to evaluate the effects of the added jujube pulp on the quality characteristics and antioxidant activities of goat milk yogurt (GMY) during 28 days of refrigerated storage. Four GMY formulations were prepared, each varying in the added jujube pulp amount (Y0: not containing jujube pulp; YJ3, YJ6, YJ9: containing 3, 6, 9 g of jujube pulp per 100 g GMY, respectively). There was no significant differences in the viable counts, pH values and titratable acidities of all formulations during the storage.

Influence of Bactrian camel milk on the gut microbiota.

Bactrian camel milk has become popular in the market as an important source of nutrients with diverse functional effects. In this study, the influence of Bactrian camel milk on the gut microbiota of mice was studied using metagenomic-based sequencing of the V3 and V4 hypervariable regions of the 16S rRNA gene. Bioinformatics analysis showed that Firmicutes and Bacteroidetes were the predominant phyla, accounting for more than 80% of the bacteria present.

Effects of ZnSO and Zn-EDTA broadcast or banded to soil on Zn bioavailability in wheat (Triticum aestivum L.) and Zn fractions in soil.

Human Zn deficiency is prevalent in developing countries, and staple grains are commonly bio-fortified to increase their Zn contents. We measured Zn content, distribution, and bioavailability in calcareous soil and in wheat plants (Triticum aestivum L.) in Shaanxi Province, China, when either an organic Zn-ethylenediaminetetraacetate (Zn-EDTA) or an inorganic zinc sulfate heptahydrate (ZnSO·7HO) Zn source was banded below the seedbed or broadcasted into soil.

Whole-genome sequencing reveals the mechanisms for evolution of streptomycin resistance in Lactobacillus plantarum.

In this research, we investigated the evolution of streptomycin resistance in Lactobacillus plantarum ATCC14917, which was passaged in medium containing a gradually increasing concentration of streptomycin. After 25 d, the minimum inhibitory concentration (MIC) of L. plantarum ATCC14917 had reached 131,072 µg/mL, which was 8,192-fold higher than the MIC of the original parent isolate.

Double Biocatalysis Signal Amplification Glucose Biosensor Based on Porous Graphene.

Controllable preparation of nanopores to promote the performance of electrochemical biosensing interfaces has become one of the researching frontiers in biosensing. A double biocatalysis signal amplification of glucose biosensor for the study of electrochemical behaviors of glucose oxidase (GOx) was proposed by using horseradish peroxidase biosynthesized porous graphene (PGR) as the platform for the biocatalytic deposition of gold nanoparticles (AuNPs). The biosensor showed a linear range from 0.

Protective effects of theasinensin A against carbon tetrachloride-induced liver injury in mice.

Theasinensins have been identified as a major group of unique catechin dimers mainly found in oolong tea and black tea. Among several types of theasinensins, theasinensin A (TSA), an epigallocatechin gallate (EGCG) dimer with an R-biphenyl bond, is the most abundant theasinensin prevalent in oolong tea. Previous studies have reported that TSA exhibits antioxidative, anti-inflammatory and anti-cancer activities in vitro and in vivo.