Visual codon: a user-friendly Python program for viewing and optimizing gene GC content.

Due to the codon bias of different species, codon optimization is usually carried out in the process of heterologous protein expression. At present, there are a variety of codon optimization tools. However, the optimized sequences may still have high or low points of local guanine and cytosine (GC) content, which is not conducive to the primer design of gene subcloning, and also makes it difficult to perform the experiment of synthesizing the whole gene with DNA fragments by polymerase chain reaction (PCR) reaction.

A Python program to merge Sanger sequences: an update.

Gene cloning is an important step in investigating gene structure and function. To verify gene sequence, Sanger sequencing is used, which may produce several overlapping sequencing files that need to be merged before alignment to the target gene sequence is performed. Previously, we reported the Python program to Merge Sanger sequences (https://peerj.

Gene synthesis design: a pythonic approach.

Researchers often need to synthesize genes of interest in this era of synthetic biology. Gene synthesis by PCR assembly of multiple DNA fragments is a quick and economical method that is widely applied. Up to now, there have been a few software solutions for designing fragments in gene synthesis.

A Python script to design primers for overlap extension PCR to ligate two DNA fragments.

Ligating two or more DNA fragments is a regular operation for the subcloning and the engineering of vectors. The overlap extension PCR serves as a straightforward method to solve this issue. However, it takes a relatively long time to design the appropriate overlapping primers and the primers for the full-length sequence, and there has not been a professional offline software for such kind of primer design.

Study on the Adsorption Mechanism of Cobalt and Nickel in Manganese Sulfate by δ-MnO.

Manganese has excellent performance in removing metal ions from aqueous solutions, but there are few studies on the adsorption and removal of heavy metal impurities in metal salt solutions. In this paper, the adsorption of cobalt and nickel ions in MnSO solution by δ-MnO prepared from two different manganese sources was studied. The optimum adsorption conditions were as follows: When the concentration of Mn was 20 g/L, δ-MnO addition was 10 g/L, Co concentration was 80 mg/L, Ni concentration was 80 mg/L, reaction time was 60 min, reaction temperature was 80 °C, and pH value was 7, the adsorption rate of Co and Ni reached more than 80%.

Comparative proteomic analysis of the sweetpotato provides insights into response mechanisms to Fusarium oxysporum f. sp. batatas.

The Fusarium wilt disease caused by Fusarium oxysporum f. sp. batatas (Fob) is one of the devastating diseases of sweetpotato.

Differential proteomic analysis of temperature-induced autolysis in mycelium of Pleurotus tuber-regium.

Autolysis is an important physiological process found in fungal cultivation. However, there is hitherto no report on the autolysis of Pleurotus tuber-regium. We have investigated the enzymes secreted by temperature-induced (40°C as treatment versus 10°C as control) autolysis of the mycelium of P.