[Effect of insulin on expression of whole-blood platelet membrane P-selectin].

Objective: To investigate the effects of insulin on the expression of whole-blood platelet membrane P-selectin.

Methods: Flow cytometry (FCM) was used to detect the expression of whole-blood platelet membrane P-selectin, and the effect of insulin on the expression on quiescent and activated plasma platelet observed in normal young subjects.

Results: Insulin had no effect on the P-selectin expression on quiescent platelet membrane, but significantly inhibited the up-regulation of P-selectin expression induced by thrombin and collagen respectively, exhibiting dose- and time-dependent effects.

[Effect of insulin on the expression of platelet membrane glycoprotein II b/III a and its mechanisms].

Objective: To investigate the effects of insulin on the expression of platelet membrane glycoprotein (GP) IIb/III a and its mechanism in normal subjects.

Methods: Flow cytometry was used to detect the expression of the whole-blood platelet membrane GP b/ a. The respective effects of insulin, L-nitro-arginine methyl ester (L-NAME) and methylene blue (MB) and their combination on the expression of GP b/ a were also studied.