A new pyrazoline-based probe of quenched fluorescent reversible recognition for Cu and its application in cells.

A new pyrazoline-based probe D was designed and synthesized, which can be used as a highly sensitive, selective and reversible recognizing fluorescent to detect Cu. The recognition properties of this compound was investigated by UV-vis absorption and fluorescence spectrophotometry. The results showed that the probe D forms a 1:1 complex with Cu and displayed a linear fluorescence response to Cu with a detection limit of 1.