Protective Effects of Ligustroflavone, an Active Compound from Ligustrum lucidum, on Diabetes-Induced Osteoporosis in Mice: A Potential Candidate as Calcium-Sensing Receptor Antagonist.

Ligustroflavone is one major compound contained in active fraction from Fructus Ligustri Lucidi (the fruit of Ligustrum lucidum), which could regulate parathyroid hormone (PTH) levels and improve calcium balance by acting on calcium-sensing receptors (CaSR). This study aimed to explore the potency of ligustroflavone as a CaSR antagonist and its protective effects against diabetic osteoporosis in mice. LF interacted well with the allosteric site of CaSR shown by molecular docking analysis, increased PTH release of primary parathyroid gland cells and suppressed extracellular calcium influx in HEK-293 cells.

Colocation of the Polymyxin Resistance Gene and a Variant of on a Plasmid in an Escherichia coli Isolate from a Chicken Farm.

A colistin-resistant isolate from a commercial poultry farm in China carried two colistin resistance genes, and variant of , in an IncP plasmid. The variant of the gene, named , encoded two amino acid substitutions compared with the gene. A novel genetic structure, IS-IS, might be the key element mediating the translocation of through the formation of a circular form.

Tn, a Novel Multidrug Resistance Transposon Characterized in a Proteus mirabilis Isolate from Chicken in China.

A novel 65.8-kb multidrug resistance transposon, designated Tn, was characterized in a isolate from chicken in China. Tn contains 18 different antimicrobial resistance genes, including cephalosporinase gene and fluoroquinolone resistance genes and ' It carries a class 1/2 hybrid integron composed of and a 3' conserved segment of the class 1 integron.

Colocation of the Multiresistance Gene and the Fosfomycin Resistance Gene on a Novel Plasmid in Staphylococcus arlettae from a Chicken Farm.

The novel 63,558-bp plasmid pSA-01, which harbors nine antibiotic resistance genes, including , (C), (L), (T), , , , , and (B), was characterized in strain SA-01, isolated from a chicken farm in China. The colocation of and genes was detected for the first time in an plasmid. The detection of two IS-mediated circular forms containing resistance genes in SA-01 suggested that IS may facilitate dissemination of antibiotic resistance genes.

A novel type 1/2 hybrid IncC plasmid carrying fifteen antimicrobial resistance genes recovered from Proteus mirabilis in China.

IncC plasmids are of great concern as vehicles of broad-spectrum cephalosporins and carbapenems resistance genes bla and bla. The aim of this study was to sequence and characterize a multidrug resistance (MDR) IncC plasmid (pPm14C18) recovered from Proteus mirabilis. pPm14C18 was identified in a CMY-2-producing P.

Colistin Resistance Gene and Its Variant in Escherichia coli Isolates from Chickens in China.

The gene was detected in 5.11% (58/1136) of isolates of chicken origin from 13 provinces in China. A novel variant, named , encoding an Ile-to-Val functional variant of MCR-1 was identified in a sequence type 155 (ST155) strain.

Various Sequence Types of Escherichia coli Isolates Coharboring and Genes from a Commercial Swine Farm in China.

Sixteen different sequence types (STs) of isolates from a commercial swine farm in China were confirmed to coharbor the carbapenem resistance gene and the colistin resistance gene Whole-genome sequencing revealed that and were located on a 46-kb IncX3 plasmid and a 32-kb IncX4 plasmid, respectively. The two plasmids can transfer together with a low fitness cost, which might explain the presence of various STs of coharboring and .

Characterization of SXT/R391 Integrative and Conjugative Elements in Proteus mirabilis Isolates from Food-Producing Animals in China.

SXT/R391 integrative and conjugative elements (ICEs) were detected in 8 out of 125 Proteus mirabilis isolates from food-producing animals in China. Whole-genome sequencing revealed that seven ICEs were identical to ICEPmiJpn1, carrying the cephalosporinase gene blaCMY-2. Another one, designated ICEPmiChn1, carried five resistance genes.

Two novel Salmonella genomic island 1 variants in Proteus mirabilis isolates from swine farms in China.

Four different Salmonella genomic island 1 (SGI1) variants, including two novel variants, were characterized in one Salmonella enterica serovar Rissen sequence type ST1917 isolate and three Proteus mirabilis isolates from swine farms in China. One novel variant was derived from SGI1-B with the backbone gene S021 disrupted by a 12.72-kb IS26 composite transposon containing the dfrA17-aadA5 cassettes and macrolide inactivation gene cluster mphA-mrx-mphR.

Molecular characteristics of Salmonella genomic island 1 in Proteus mirabilis isolates from poultry farms in China.

Six out of the 64 studied Proteus mirabilis isolates from 11 poultry farms in China contained Salmonella genomic island 1 (SGI1). PCR mapping showed that the complete nucleotide sequences of SGI1s ranged from 33.2 to 42.

[Effects of temperature, salinity, and light intensity on the growth and toxin production of Chattonella marina].

A laboratory experiment with orthogonal design was conducted to study the effects of factors salinity, temperature, and light intensity on the growth and toxin production of Chattonella marina. Three levels of salinity (22, 33, and 45), temperature (20 degrees C, 25 degrees C, and 30 degrees C) and light intensity (2000, 3000, and 4500 lx) were installed. In all treatments, the three factors had no significant effects on the growth of C.