- and -Palmitoleic Acid Isomers Regulate Cholesterol Metabolism in Different Ways.

Hypercholesterolemia is a preventable risk factor for atherosclerosis and cardiovascular disease. However, the mechanisms whereby -palmitoleic acid (POA) and -palmitoleic acid (POA) promote cholesterol homeostasis and ameliorate hypercholesterolemia remain elusive. To investigate the effects of POA and POA on cholesterol metabolism and its mechanisms, we induced hypercholesterolemia in mice using a high-fat diet and then intragastrically administered POA or POA once daily for 4 weeks.

Comparing the simultaneous determination of cis- and trans-palmitoleic acid in fish oil using HPLC and GC.

Background: Cis- and trans-palmitoleic acids (Cis-POA and trans-POA) are isomers of palmitoleic acid, a monounsaturated fatty acid which affects glucose and lipid metabolism, and reduces insulin resistance. Trans-POA is used as a biomarker for indicating the risk of type II diabetes and coronary heart disease, but no methods of analysis or distinguishing between cis-POA and trans-POA have yet been reported.

Method: An accurate and precise HPLC method was developed to determine cis- and trans-POA simultaneously, and compared with results from a GC method.