Lentilactobacillus farraginis FSI (3): a whole cell biocatalyst for the synthesis of kojic acid derivative under aquatic condition.

Kojic acid derivatives are useful in the cosmetics and pharmaceutical industries. The current investigation focuses on the search for a safe and environmentally friendly newer whole-cell biocatalyst for the synthesis of kojic acid derivative especially 2-amino-6-(hydroxymethyl)-8-oxo-4-phenyl-4,8-dihydropyrano[3,2-b]pyran-3-carbonitrile (APhCN). In this context, a total of six cultures were isolated from fecal samples of infants and subjected to probiotic characterization followed by screening as whole cell biocatalyst (WCB).

Lipase-mediated multicomponent synthesis of 1H-Pyrazolo[1,2-b]phthalazine-5,10-dione derivatives in a binary solvent medium.

A new method for the synthesis of 1H-pyrazolo[1,2-b]phthalazine-5,10-dione derivatives via lipase from the Aspergillus niger-catalyzed multicomponent reaction of aldehydes, malononitrile, and phthalhydrazide is reported herein for the first time. This novel method holds several advantages, including its efficiency, environmental friendliness, simple workup procedure, and good yield (70-86%). The effects of temperature, organic solvents, and water content were investigated.

Effect of CD6 on serum lipid profile and indicators of liver function in high-fat diet fed Swiss albino mice.

In this study, we have investigated the effect of CD6 on weight gain, lipid profile, liver function biomarkers (ALT: alanine aminotransferase; and AST: aspartate aminotransferase) and liver histopathology in high-fat diet fed Swiss albino mice. Twenty-four healthy male Swiss albino mice with an average body weight of 25.94 ± 0.

A halotolerant hyaluronidase from newly isolated Brevibacterium halotolerans DC1: Purification and characterization.

An enzyme hyaluronidase (hyase) producing halotolerant bacterium was isolated from dental caries and identified as Brevibacterium halotolerans DC1. Higher growth and hyase production were observed in nutrient broth fortified with hyaluronic acid at pH 7.0, temperature 37 °C, 120 rpm upon 48 h of incubation.

Correlation of motivations for selfie-posting behavior with personality traits.

Background: The widespread popularity of selfie-posting behavior has led to increasing academic interest in exploring psychological determinants for this behavior.

Aim: The study aimed to evaluate sociodemographic factors, personality traits, and psychological motivations associated with selfie-posting behavior and to evaluate the association of psychological motivations for selfie-posting with personality traits.

Materials And Methods: This cross-sectional study assessed 727 medical and physiotherapy students with a semi-structured questionnaire consisting of information about sociodemographic factors, selfie-posting, and editing behavior, the Big Five Inventory and motivations for selfie-posting scale.

Bioprocess optimization of laccase production through solid substrate fermentation using Perenniporia tephropora-L168 and its application in bioremediation of triaryl-methane dye.

Laccases are multi copper oxidases that can oxidize both phenolic and nonphenolic lignin related compounds. Consequently, there has been continuous demand for laccases for the oxidative degradation of phenolic dyes in effluents. In view of this, the present work was focused on laccase production by solid substrate fermentation using a newly isolated fungus Perenniporia tephropora-L168.

Instant green synthesis of silver-based herbo-metallic colloidal nanosuspension in Terminalia bellirica fruit aqueous extract for catalytic and antibacterial applications.

In the present study, microwave-assisted, optimized, instant, Terminalia bellirica fruit extract-mediated green synthesis of colloidal silver nanoparticles (AgNPs) has been reported. The synthesized AgNPs were characterized by UV-Vis spectroscopy, FTIR, Zetasizer, FESEM, EDX and XRD. The characteristic surface plasmon peak of reaction mixture at 406 nm confirmed the synthesis of AgNPs.

Bio-therapeutic Potential and Cytotoxicity Assessment of Pectin-Mediated Synthesized Nanostructured Cerium Oxide.

In the present studies, renewable and nontoxic biopolymer, pectin, was extracted from Indian red pomelo fruit peels and used for the synthesis of cerium oxide nanoparticles (CeO-NPs) having bio-therapeutic potential. The structural information of extracted pectin was investigated by FTIR and NMR spectroscopic techniques. Physicochemical characteristics of this pectin suggested its application in the synthesis of metal oxide nanoparticles.

Selective ciprofloxacin antibiotic detection by fluorescent siderophore pyoverdin.

Fluorescent siderophore pyoverdin (PVD) was produced from a soil isolate Pseudomonas monteilii strain MKP 213. The PVD was purified near to homogeneity and applied for the fluorescent chemosensing of various antibiotics in aqueous solution (pH=7.0).

Antioxidative potential of folate producing probiotic Lactobacillus helveticus CD6.

Folate producing Lactobacillus sp. CD6 isolated from fermented milk showed 98% similarity with Lactobacillus helveticus based on 16S rRNA gene sequence analysis. It was found to produce a folic acid derivative 5-methyl tetrahydrofolate (5-MeTHF).

Production, Purification of Exo-Polygalacturonase from Soil Isolate Paecilomyces variotii NFCCI 1769 and Its Application.

The aim of the present study was to produce exo-polygalacturonase from potent soil isolate by submerged fermentation and its application for fruit juice treatment. Pectinase producing strains were selectively isolated from pectin industry waste. A selected isolate C2 was found to produce significant amount of exo-polygalacturonase.

Cholesterol assimilation and biotransformation by Lactobacillus helveticus.

Lactobacillus helveticus, grown at 37°C in MRS medium supplemented with 3 mM cholesterol, assimilated all the cholesterol in 42 h having 68 U mg(-1) of intracellular cholesterol oxidase activity. The strain transformed 1 g cholesterol to 0.05 g of androsta-1, 4-diene-3, 17-dione and 0.

A potential probiotic culture ST2 produces siderophore 2,3-dihydroxybenzoylserine under intestinal conditions.

The aim of this study was to characterise siderophores of a culture bearing better probiotic qualities of human origin. In this direction, bacterial strain ST2 was isolated from faecal sample of 6-month-old healthy infant, which showed close similarity with Escherichia coli based on 16S r-RNA gene sequence analysis and biochemical characteristics. It was tested for siderophore production under in vitro iron limiting intestinal conditions.

Bacillus spp. of human origin: a potential siderophoregenic probiotic bacteria.

Bacillus spp. ST13, isolated from human stool, was evaluated for siderophoregenic and probiotic qualities prior to its possible application for iron nutrition in humans and animals. It was tested for siderophore production in iron-limiting conditions and found to produce catecholate type of siderophore on the basis of high-performance liquid chromatography (HPLC), FT-IR, NMR, and mass spectra analysis.

Production of hyaluronic acid from Streptococcus zooepidemicus MTCC 3523 and its wound healing activity.

Exopolysaccharide produced and purified from Streptococcus zooepidemicus MTCC 3523 was identified as hyaluronic acid (HA) based on IR and NMR spectroscopy while its Mw was found to be 5.38 × 10(5)Da. HA produced passed bacterial endotoxin test and showed significant wound healing activity in Wistar rats on 12th and 16th day.

Cholesterol biotransformation to androsta-1,4-diene-3,17-dione by growing cells of Chryseobacterium gleum.

Cholesterol oxidase activity was studied during biotransformation of cholesterol to androsta-1,4-diene-3,17-dione (ADD) by Chryseobacterium gleum. Spent LB media, containing cholesterol (3 mM approximately = 1 g l(-1)) where the bacterium was grown for 24 h, at 30 degrees C with constant shaking at 120 rpm, had the highest enzyme activity (167 U mg(-1)). The growing cells produced 0.

Evaluation of probiotic characteristics of siderophoregenic Bacillus spp. isolated from dairy waste.

Siderophoregenic Bacillus strain DET9 has been selectively isolated from dairy waste. It was evaluated for probiotic characteristics and susceptibility pattern against antibiotics. Its spores showed excellent tolerance to simulated gastrointestinal tract conditions and exhibited antimicrobial activity against organisms such as Escherichia coli, Micrococcus flavus, and Staphylococcus aureus.

Characteristics of sulfobacin A from a soil isolate Chryseobacterium gleum.

A nonmotile, nonspore-forming, Gram-negative, aerobic, small rod-shaped bacterium, isolated from soil, was identified as Chryseobacterium gleum on the basis of 16S rRNA gene sequence analysis. It was observed to grow luxuriously at pH 9 and tolerate highly alkaline environment up to pH 12. Orange red color was a peculiar character of these cells which on purification obtained 60-80 mg/l and found to be sphingosine type of sulfonolipid "sulfobacin A" on the basis of infrared, nuclear magnetic resonance, and mass spectral data.

Exploring antagonistic metabolites of established biocontrol agent of marine origin.

Biocontrol ability of Pseudomonas aeruginosa ID 4365, a biocontrol agent of groundnut phytopathogens from marine origin, was previously attributed to the production of pyoverdin type of siderophores. However, pyoverdin-rich supernatants of this organism showed better antifungal activity compared to equivalent amount of purified pyoverdin indicating presence of undetected metabolite(s) in pyoverdin rich supernatants. On the basis of observation that antagonistic activity was iron-dependent and iron-independent, an attempt was made to detect the presence of additional metabolites.

Production, purification and chemical characterization of the catecholate siderophore from potent probiotic strains of Bacillus spp.

The aim of the present study was to characterize the probiotic qualities of Bacillus isolates and study their siderophore prior to possible siderophoregenic probiotic application for iron nutrition in animals and humans. Bacillus strains were selectively isolated from dairy waste and mango pulp waste. Best two siderophore positive isolates, JHT3 and DET6 showed high homology with Bacillus megaterium (98%) and B.