Investigation of non-CB, non-CB WIN55212-2-sensitive G-protein-coupled receptors in the brains of mammals, birds, and amphibians.

Purpose: Previous studies have found non-CB non-CB G-protein-coupled receptors in rodents that are activated by the aminoalkylindole cannabinoid agonist WIN55212-2. This work obtained evidence for the presence or absence of similar receptors in the brains of other mammals, birds and amphibians.

Materials And Methods: Antagonism of the stimulation of [S]GTPγS binding by WIN55212-2 and CP55940 was assessed in multiple CNS regions of rat and canine, and in whole brain membranes from shrew, pigeon, frog and newt.

Preparation and characterization of a compounded aqueous human insulin suspension.

Human insulin, used in the management of diabetes, is commercially available in the form of solutions or suspensions for injection. This study investigated the preparation and characterization of an oral suspension for insulin. The suspension was prepared by adding human insulin to aqueous soy extract and adjusting the pH of the preparation to 4.