Publications by authors named "Bhavana Shewale"
Article Synopsis
- Cardiac Purkinje fibers are crucial for coordinating heart contractions and understanding their biology is limited due to challenges in creating these cells from human stem cells.
- Researchers analyzed single-cell data from mouse hearts to identify key signaling pathways, such as Notch signaling, that are involved in the formation of Purkinje fiber cells.
- Activating Notch signaling in human embryonic stem cell-derived cardiomyocytes not only transformed their shape and electrical properties to resemble Purkinje fibers but also altered their gene expression, resulting in lower contractile force and differing responses to anti-arrhythmogenic drugs.
Article Synopsis
- A study investigates how drug-induced gene expression profiles can reveal mechanisms of cardiotoxicity in FDA-approved tyrosine kinase inhibitors (TKIs) using human stem cell-derived heart cells.
- The research employs singular value decomposition to detect drug-specific patterns in cells from various healthy individuals, highlighting affected cellular pathways like energy metabolism and contractile functions.
- The findings suggest that integrating mRNA expression data with genomic and pathway information can create comprehensive signatures for cardiotoxicity, aiding in drug development and personalized treatment strategies.
Pluripotent stem-cell-derived cardiomyocytes (PSC-CMs) provide an unprecedented opportunity to study human heart development and disease, but they are functionally and structurally immature. Here, we induce efficient human PSC-CM (hPSC-CM) maturation through metabolic-pathway modulations. Specifically, we find that peroxisome-proliferator-associated receptor (PPAR) signaling regulates glycolysis and fatty acid oxidation (FAO) in an isoform-specific manner.
View Article and Find Full Text PDFCraniofacial development depends on formation and maintenance of sutures between bones of the skull. In sutures, growth occurs at osteogenic fronts along the edge of each bone, and suture mesenchyme separates adjacent bones. Here, we perform single-cell RNA-seq analysis of the embryonic, wild type murine coronal suture to define its population structure.
View Article and Find Full Text PDFOf form and function: Early cardiac morphogenesis across classical and emerging model systems.
Semin Cell Dev Biol
October 2021
The heart is the earliest organ to develop during embryogenesis and is remarkable in its ability to function efficiently as it is being sculpted. Cardiac heart defects account for a high burden of childhood developmental disorders with many remaining poorly understood mechanistically. Decades of work across a multitude of model organisms has informed our understanding of early cardiac differentiation and morphogenesis and has simultaneously opened new and unanswered questions.
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