Electrophysiological studies in children with paralytic poliomyelitis.

Ten healthy children (Group I), twenty children with acute spinal poliomyelitis (Group II and III) and twenty children of post-polio residual paralysis (Group IV) between the age group of 9 months to 4 years were the study subjects. The motor nerve conduction velocity (MNCV), standardized distal motor latency (SDML) and motor latency (M-RL) were similar in all the groups studied No H-reflex could be elicited in children with acute spinal poliomyelitis seen within one week of onset of paralysis (Group II). The H-max from 4-6 week group (III) improved with time in 9-12 month (Group IV) but did not attain the control (Group I) value.

Detection and purification of sequence-specific DNA binding protein.

A simple assay for identifying DNA-binding proteins is described that involves loading of protein fractions onto nitrocellulose membrane using a slot-blot apparatus, incubating with 32P-labeled DNA probe in buffer in the presence of excess of nonspecific E. coli DNA at room temperature, and washing with increasing concentration of NaCl (from 50 to 500 mM) to obtain optimum signal. A simple and rapid scheme of purification of a sex and tissue-specific DNA-binding protein, which binds specifically to the GATA repeats of Bkm (banded krait minor satellite DNA), designated as Bkm-binding protein (BBP), is also described.

A single-step immunochromatographic test for the detection of Entamoeba histolytica antigen in stool samples.

A rapid, single step, sensitive and specific immunochromatographic test for the detection of E. histolytica antigens in the stool samples is described. Monoclonal antibody AC55 specific for E.

Slide agglutination test for the diagnosis of pulmonary and extra-pulmonary tuberculosis.

Objective: Evaluation of a 2 min slide agglutination test to detect the presence of antibodies directed against Mycobacterium tuberculosis antigens.

Design: The test utilizes solible antigens extracted from a cultivable new species of non-pathogenic saprophytic mycobacterium, Mycobacterium w, which shares antigenic determinants with M. tuberculosis.

Four steps to soft tissue management: non-surgical periodontal therapy.

Soft tissue management or nonsurgical periodontal therapy is based upon the current concepts of the dental plaque and the nature of periodontal disease progression. It is the initial step in the management of adult periodontitis and in those patients who, for what ever reasons, do not wish surgery, it is an excellent second choice of therapy. STM can be done by the general dentist and/or the hygienist.

Subclavian vein catheters for haemodialysis with and without a subcutaneous tunnel.

We have retrospectively analysed the usefulness of a subcutaneous tunnel in patients undergoing haemodialysis through a double lumen subclavian catheter; 194 catheters were used in 145 patients. In 105 patients a subcutaneous tunnel was created. Entry site infection was significantly higher in the no tunnel group (30%; P < 0.

Management of childhood femoral neck fractures.

Forty-three children with displaced transcervical femoral fractures were reviewed at a mean follow-up of 7.2 years and results were assessed using Ratliff's criteria. Of 22 patients treated by internal fixation alone, 10 had good, 6 had fair and 6 had poor results.

Short term study on relative merits of acetate and bicarbonate dialysis.

We did a double blind, crossover, prospective study comparing bicarbonate and acetate containing solutions in haemodialysis. Thirty stable patients with end stage renal disease on maintenance haemodialysis while awaiting a renal transplant were each studied on three dialysis with acetate and three with bicarbonate. Nine patients developed symptoms like headache, nausea, vomiting, giddiness, and malaise and developed hypotension during acetate dialysis and three patients during bicarbonate dialysis.

An enzymatic method for TLC detection and determination of fenitrothion in water.

A simple enzymatic method is described for field TLC detection and determination of fenitrothion as fenitrooxon in water, with pig liver acetone powder as enzyme source. By this method, fenitrothion can be detected as fenitrooxon at ng levels and amounts ranging from 5 to 50 ng can be estimated.

Colorimetric determination of dimethoate or omethoate.

A simple, sensitive and rapid colorimetric method is described for determining dimethoate [O,O-dimethyl-S-(N-methylcarbanoylmethyl)phosphorodithioate] and omethoate by an enzymatic method using pig liver acetone powder as enzyme source and p-nitrobenzenediazonium fluoroborate as the chromogenic reagent. This colorimetric method is more sensitive than non-enzymatic methods. Inhibition can be detected at ng levels and amounts ranging from 50 to 1000 ng of omethoate and from 1 to 10 mug of dimethoate can be estimated.