A comprehensive evaluation of Cell Elution Method (CEM) for forensic DNA analysis from smoked cigarettes.

Cigarette stubs are commonly encountered trace DNA samples at crime scenes. Standard laboratory practice typically involves direct lysis of the stub for DNA extraction, leading to the co-extraction of DNA-degrading and inhibiting constituents from smoke and tobacco. This process can result in lower-quality DNA profiles.

Forensic evaluation of mitochondrial DNA heteroplasmy in Gujarat population, India.

Background: Owing to its high copy number and its small size, mtDNA analysis is the most reliable choice when biological materials from crime scenes are degraded or have mixed STR profiles.

Aim: To examine the occurrence of heteroplasmy along with its frequency and pattern in both HV1 and HV2 regions of the mtDNA among unrelated individuals from India.

Subjects And Methods: Mitochondrial DNA control region [hypervariable region one (HV1) and hypervariable region two (HV2)] were analysed in blood and buccal tissues of 104 unrelated individuals from the Indian state of Gujarat.

Comparative evaluation of different human dental tissues and alveolar bone for DNA quantity and quality for forensic investigation.

In this study, the efficacy of dental tissues (cementum, dentine and pulp) and alveolar bone as a potential source of DNA was tested in terms of the quality and quantity using nuclear and mitochondrial markers for forensic investigation.This study found dentine as the best source of DNA with only 5.36% imbalanced (PHR<0.

A systematic evaluation of 'Bidi - a hand-rolled cigarette' as a forensic DNA evidence.

Bidis are small handmade cigarettes consisting of ~0.2 g of tobacco flakes rolled in a dried leaf of 'Tendu' (Diospyros melanoxylon) or Piliostigma racemosum and tied with a thin thread. They have gained worldwide popularity among smokers and are often collected as forensic DNA evidence from crime scenes and processed similarly to cigarette butts.

Kinetics and mechanisms of mercury biosorption by an exopolysaccharide producing marine isolate .

Eight exopolysaccharide (EPS) producing metal-removing marine bacteria were screened for mercury (Hg) sorption. with the highest MIC values and Hg sorption ability was selected for further study. Biosorption of Hg from aqueous solution by was studied with respect to the metal concentration, adsorbent concentration, pH, different contact times, and in the presence of other metal ions.

Application of novel consortium TSR for treatment of industrial dye manufacturing effluent with concurrent removal of ADMI, COD, heavy metals and toxicity.

The present study was aimed towards the effective bio-treatment of actual industrial effluent containing as high as 42,000 mg/L COD (chemical oxygen demand), >28,000 ADMI (American Dye Manufacturers Institute) color value and four heavy metals using indigenous developed bacterial consortium TSR. Mineral salt medium supplemented with as low as 0.02% (w/v) yeast extract and glucose was found to remove 70% ADMI, 69% COD and >99% sorption of heavy metals in 24 h from the effluent by consortium TSR.

A novel biphasic leaching approach for the recovery of Cu and Zn from polymetallic bulk concentrate.

In scale-up biphasic leaching process of polymetallic concentrate, the ferric bioregeneration cycles were performed in 15.0L down flow packed bed reactor; whereas the chemical leaching cycles were done using the biogenerated ferric in an indigenously designed 10.0L stirred tank reactor.