Modelling and optimization of biodiesel production from waste fish oil using nano immobilized rPichiapastoris whole cell biocatalyst with response surface methodology and hybrid artificial neural network based approach.

In this study, zinc oxide (ZnO) nano particle immobilized recombinant whole cell biocatalyst (rWCB) was used for bioconversion of waste fish oil in to biodiesel in a lab scale packed bed reactor (PBR). Central composite design and hybrid artificial neural network (ANN) models were explored to optimize the production of biodiesel. Developed rWCB exhibited maximum lipase activity at 15 % (v/v) of glutaraldehyde concentration and 6 % (w/v) of ZnO nanoparticles at pH of 7.

Energy generation from bioelectrochemical techniques: Concepts, reactor configurations and modeling approaches.

The process industries play a significant role in boosting the economy of any nation. However, poor management in several industries has been posing worrisome threats to an environment that was previously immaculate. As a result, the untreated waste and wastewater discarded by many industries contain abundant organic matter and other toxic chemicals.

Current and prognostic overview on the strategic exploitation of anaerobic digestion and digestate: A review.

The depletion of fossil fuels and increasing demand for energy are encountered by generating renewable biogas. Anaerobic digestion (AD) produces not only biogas, also other value-added products from the digestate using various organic, municipal and industrial wastes which have several benefits like remediating waste, reduces greenhouse gas emissions, renewable energy generation and securing socio-economic status of bio-based industries. This review work critically analyzes the biorefinery approaches on AD process for the production of biogas and digestate, and their direct and indirect utilization.

Production of biodiesel from Caulerpa racemosa oil using recombinant Pichia pastoris whole cell biocatalyst with double displayed over expression of Candida antartica lipase.

In this study, Caulerpa racemosa oil was used to produce biodiesel by recombinant Pichia pastoris displaying bound (rPp-BL) and secretory lipase (rPp-SL). Collected algae was pre-treated using ultrasonication, microwave and solvent extraction. Defatted C.

Techno economic analysis of malic acid production using crude glycerol derived from waste cooking oil.

In the present work, Aspergillus niger was employed to produce commercially valuable malic acid from crude glycerol derived from waste cooking oil. Crude glycerol dosage, yeast extract dosage and initial pH were the influencing factors playing a significant role in the malic acid synthesis. The optimal condition for malic acid biosynthesis was studied by using response surface methodology.

Overview of Current Developments in Biobutanol Production Methods and Future Perspectives.

Renewable biobutanol production is receiving more attention toward substituting fossil-based nonrenewable fuels. Biobutanol is recognized as the top most biofuel with extraordinary properties as compared with gasoline. The demand for biobutanol production is increasing enormously due to application in various industries as chemical substituent.

Modelling and process optimization for biodiesel production from Nannochloropsis salina using artificial neural network.

In the present investigation, calcium oxide solid nanocatalyst derived from the egg shell and Nannochloropsis salina were used for the production of biodiesel. The morphological characteristics and functional groups of synthesized nanocatalyst was characterized by SEM and FTIR analysis. Process variables optimization for biodiesel production was studied using RSM and ANN.

Green processing and biotechnological potential of grape pomace: Current trends and opportunities for sustainable biorefinery.

Grape pomace is a high quality biodegradable residue of the winery industry. It is comprised of grape seed, skin and stalks, and is blessed with substantial quantities of phenols, flavonoids and anthocyanins with high antioxidant potential. Currently, there is huge emphasis on the isolation of bioactive molecules of grape pomace using green technologies such as microwave, ultrasound, supercritical fluids, high voltage discharge, enzymatic methods and other hybrid techniques.

Enhanced malic acid production using Aspergillus niger coupled with in situ product recovery.

In the present investigation, recovery of malic acid from the fermentation broth was performed by using in situ reactive extraction method employing different combination of amine and solvent systems. Totally six solvent mixtures were tested for toxicity on Aspergillus niger PJR1. Further, effect of solvent mixture concentration on separation of malic acid was investigated.

Biochemical conversion of biodiesel by-product into malic acid: A way towards sustainability.

Crude glycerol, one of the ever-growing by-product of biodiesel industry and is receiving the closest review in recent times because direct disposal of crude glycerol may emerge ecological issues. The renewability, bioavailability and typical structure of glycerol, therefore, discover conceivable application in serving the role of carbon and energy source for microbial biosynthesis of high value products. This conceivable arrangement could find exploitation of crude glycerol as a renewable building block for bio-refineries as it is economically as well as environmentally profitable.

Bioethanol production from woody stem Prosopis juliflora using thermo tolerant yeast Kluyveromyces marxianus and its kinetics studies.

In the present study, Kluyveromyces marxianus was utilized to study the batch fermentation kinetics of biomass production, substrate utilization and bioethanol production from woody stem Prosopis juliflora. The pre-treated substrate was subjected to Simultaneous Saccharification and Fermentation (SSF) under optimised conditions of pH (4.9), temperature (41 °C), substrate concentration 5% (w/v), inoculum concentration 3% (v/v) and the maximum concentration of bioethanol was found to be 21.

Continuous production of biohydrogen from brewery effluent using co-culture of mutated Rhodobacter M 19 and Enterobacter aerogenes.

This study investigated the biohydrogen production from brewery effluents using free and immobilized co-culture of mutated Rhodobacter M 19 and Enterobacter aerogenes obtained from random mutagenesis with ultra violet (UV) and ethidium bromide (EtBr) treatment. The best mutant for biohydrogen production was screened based on the sugar utilization efficiency. Maximum hydrogen production of 87% was achieved with immobilized EtBr mutated co-culture.

Biodiesel production from microalgae Nannochloropsis oculata using heterogeneous Poly Ethylene Glycol (PEG) encapsulated ZnOMn nanocatalyst.

In this present work nanocomposite composed of Mn-ZnO capped with Poly Ethylene Glycol (PEG) was utilized as heterogeneous catalyst for the transesterification of oil extracted from Nannochloropsis oculata into biodiesel using methanol as an acyl acceptor. The synthesized Mn-ZnO novel nanocomposite capped with Poly Ethylene Glycol (PEG) was characterized by using SEM and XRD. Lipid contents from the microalgae were extracted by sonication and biphasic solvent method.

Process optimization and kinetic analysis of malic acid production from crude glycerol using Aspergillus niger.

In the present work, optimization of crude glycerol fermentation to produce malic acid by using Aspergillus niger was investigated using response surface methodology and artificial neural network. Kinetic investigation of bioconversion of crude glycerol into malic acid using Aspergillus niger was studied using Monod, Mosser, and Haldane-Andrew models. Crude glycerol concentration, initial pH and yeast extract concentration were found to be significant compounds affecting malic acid production by Aspergillus niger.

Malic acid production from biodiesel derived crude glycerol using morphologically controlled Aspergillus niger in batch fermentation.

In the present investigation, the effects of crude glycerol concentration, spore inoculum concentration, yeast extract concentration and shaking frequency on seed morphology of Aspergillus niger PJR1 on malic acid production were investigated and dispersed fungal mycelium with higher biomass (20.25 ± 0.91 g/L) was obtained when A.

Malic acid production by chemically induced Aspergillus niger MTCC 281 mutant from crude glycerol.

In the present investigation, crude glycerol derived from transesterification process was utilized to produce the commercially-valuable malic acid. A combined resistant on methanol and malic acid strain of Aspergillus niger MTCC 281 mutant was generated in solid medium containing methanol (1-5%) and malic acid (40-80 g/L) by the adaptation process for 22 weeks. The ability of induced Aspergillus niger MTCC 281 mutant to utilize crude glycerol and pure glycerol to produce malic acid was studied.

Biodiesel production from microbial oil derived from wood isolate Trichoderma reesei.

In the present study Trichoderma reesei, a wood isolate can yield high biomass quantities up to 30g/L, yielding 32.4% of lipids of dry cell weight (DCW). Biodiesel production from Trichoderma reesei involved simple unit operations like filtration and ultrasonication, yet giving good lipid yield with desirable bio-diesel properties.

Microbial oil - A plausible alternate resource for food and fuel application.

Microbes have recourse to low-priced substrates like agricultural wastes and industrial efflux. A pragmatic approach towards an emerging field- the exploitation of microbial oils for biodiesel production, pharmaceutical and cosmetic applications, food additives, biopolymer production will be of immense remunerative significance in the near future. Due to high free fatty acid, nutritive content and simpler solvent extraction processes of microbial oils with plant oil, microbial oils can back plant oils in food applications.

Biodiesel production from different algal oil using immobilized pure lipase and tailor made rPichia pastoris with Cal A and Cal B genes.

In this investigation, oil extraction was performed in marine macroalgae Gracilaria edulis, Enteromorpha compressa and Ulva lactuca. The algal biomass was characterized by Scanning Electron Microscopy and Fourier Transform-Infra Red Spectroscopy. Six different pre-treatment methods were carried out to evaluate the best method for maximum oil extraction.