Effect of thawing methods on frozen semen quality of yak (Poephagus grunniens L.) bulls.

Aim: To evaluate different thawing temperatures and duration on the post-thaw semen quality of Indian yaks bulls.

Materials And Methods: Semen ejaculates from four different yak bulls were collected using artificial vagina method and extended with tris extender containing 6.4% glycerol at 35°C, cooled gradually from 35°C to 5°C at 1°C/3 min and equilibrated at 4-5°C for 4 h and frozen in French mini straws using a programmable bio-freezer and finally stored in liquid nitrogen.