Modulation of mRNA expression by the long non-coding RNA Assolrna in affects solvent formation.

Solvents such as butanol are important platform chemicals and are often produced from petrochemical sources. Production of butanol and other compounds from renewable and sustainable resources can be achieved by solventogenic bacteria, such as the hyper-butanol producer . Its operon consists of the genes encoding butyraldehyde dehydrogenase, CoA transferase, and acetoacetate decarboxylase (, , , ) and the gene products are involved in butanol and acetone formation.

Anaerobic Production of Poly(3-hydroxybutyrate) and Its Precursor 3-Hydroxybutyrate from Synthesis Gas by Autotrophic Clostridia.

Anaerobic production of the biopolymer poly(3-hydroxybutyrate) (PHB) and the monomer 3-hydroxybutyrate (3-HB) was achieved using recombinant clostridial acetogens supplied with syn(thesis) gas as the sole carbon and energy source. 3-HB production was successfully accomplished by a new synthetic pathway containing the genes (encoding thiolase A), (encoding CoA-transferase A/B), and (encoding ()-3-hydroxybutyrate dehydrogenase). The respective recombinant [p83_tcb] strain produced autotrophically 0.

Genome Sequence of the Acetogenic Bacterium Acetobacterium wieringae DSM 1911T.

Here, we report the draft genome sequence of Acetobacterium wieringae DSM 1911, an anaerobic, autotrophic, acetogenic, d,l-lactate-utilizing bacterium. The genome consists of a chromosome (3.88 Mb) and 3,620 predicted protein-encoding genes.

Genome Sequence of the Poly-3-Hydroxybutyrate Producer Clostridium acetireducens DSM 10703.

Here, we report the genome sequence of Clostridium acetireducens (DSM 10703), a strictly anaerobic bacterium capable of fermenting acetate and leucine to butyrate, isovalerate, and poly-3-hydroxybutyrate. The draft genome consists of a circular chromosome with a size of 2.4 Mb and harbors 2,239 predicted protein-encoding genes.

Genome Sequence of the Acetogenic Bacterium Butyribacterium methylotrophicum DSM 3468T.

Butyribacterium methylotrophicum DSM 3468 is an acetogenic methylotrophic, anaerobic, carbon monoxide-oxidizing bacterium that produces acetate, butyrate, and butanol. The genome consists of a single chromosome (4.3 Mb) and harbors 3,989 predicted protein-encoding genes.

Cap0037, a Novel Global Regulator of Clostridium acetobutylicum Metabolism.

Unlabelled: An operon comprising two genes, CA_P0037 and CA_P0036, that encode proteins of unknown function that were previously shown to be highly expressed in acidogenic cells and repressed in solventogenic and alcohologenic cells is located on the pSOL1 megaplasmid of Clostridium acetobutylicum upstream of adhE2 A CA_P0037::int (189/190s) mutant in which an intron was inserted at position 189/190 in the sense strand of CA_P0037 was successfully generated by the Targetron technique. The resultant mutant showed significantly different metabolic flux patterns in acidogenic (producing mainly lactate, butyrate, and butanol) and alcohologenic (producing mainly butyrate, acetate, and lactate) chemostat cultures but not in solventogenic or batch cultures. Transcriptomic investigation of the CA_P0037::int (189/190s) mutant showed that inactivation of CA_P0037 significantly affected the expression of more than 258 genes under acidogenic conditions.

Draft Genome Sequence of the Strict Anaerobe Clostridium neopropionicum X4 (DSM 3847T).

Here, we report the draft genome sequence ofClostridium neopropionicumX4 (DSM 3847(T)), a strictly anaerobic bacterium capable of fermenting ethanol and CO2to propionate, acetate, and propanol. The genome consists of a single chromosome (3.19 Mb).

Genome Sequence of the Acetogenic Bacterium Oxobacter pfennigii DSM 3222T.

Here, we report the draft genome sequence of Oxobacter pfennigii DSM 3222(T), an anaerobic, acetogenic, carbon monoxide-oxidizing, and butyrate-producing bacterium. The genome consists of a chromosome with a size of 4.49 Mbp.

Complete Genome Sequence of the Type Strain of the Acetogenic Bacterium Moorella thermoacetica DSM 521T.

Here we report the closed genome sequence of the type strain Moorella thermoacetica DSM 521(T), an acetogenic bacterium, which is able to grow autotrophically on H2 + CO2 and/or CO, using the Wood-Ljungdahl pathway. The genome consists of a circular chromosome (2.53 Mb).

Complete Genome Sequence of the Acetogenic Bacterium Moorella thermoacetica DSM 2955T.

Here, we report the complete genome sequence of Moorella thermoacetica DSM 2955(T), an acetogenic bacterium, which uses the Wood-Ljungdahl pathway for reduction of H2 + CO2 or CO. The genome consists of a single circular chromosome (2.62 Mb).

Draft Genome Sequence of the Strict Anaerobe Clostridium homopropionicum LuHBu1 (DSM 5847).

Here, we report the draft genome sequence of Clostridium homopropionicum LuHBu1 (DSM 5847(T)), a strictly anaerobic bacterium, which performs propionate fermentation and is capable of growing with 2-, 3-, or 4-hydroxybutyrate as its sole substrate. The genome consists of a single chromosome of 3.65 Mb.

Draft Genome Sequence of Purine-Degrading Gottschalkia purinilyticum (Formerly Clostridium purinilyticum) WA1 (DSM 1384).

Here, we report the draft genome sequence of Gottschalkia purinilyticum (formerly Clostridium purinilyticum) WA1, an anaerobic bacterium specialized on degradation of purines (including adenine) and glycine, which uses the selenoprotein glycine reductase for substrate degradation. The genome consists of a single chromosome (3.40 Mb).

The Complete Genome Sequence of Clostridium aceticum: a Missing Link between Rnf- and Cytochrome-Containing Autotrophic Acetogens.

Unlabelled: Clostridium aceticum was the first isolated autotrophic acetogen, converting CO2 plus H2 or syngas to acetate. Its genome has now been completely sequenced and consists of a 4.2-Mbp chromosome and a small circular plasmid of 5.

Draft Genome Sequence of Purine-Degrading Clostridium cylindrosporum HC-1 (DSM 605).

Here, we report the draft genome sequence of Clostridium cylindrosporum HC-1, a purine- and glycine-fermenting anaerobe, which uses selenoprotein glycine reductase for substrate degradation. The genome consists of a single chromosome (2.72 Mb) and a circular plasmid (14.

Complete Genome Sequence of Rnf- and Cytochrome-Containing Autotrophic Acetogen Clostridium aceticum DSM 1496.

Here, we report the closed genome sequence of Clostridium aceticum, an Rnf- and cytochrome-containing autotrophic acetogen that is able to convert CO2 and H2 to acetate using the Wood-Ljungdahl pathway. The genome consists of a circular chromosome (4.2 Mbp) and a small circular plasmid (5.

Butanol fermentation.

This review provides an overview on bacterial butanol production and recent developments concerning strain improvement, newly built butanol production plants, and the importance of alternative substrates, especially lignocellulosic hydrolysates. The butanol fermentation using solventogenic clostridial strains, particularly Clostridium acetobutylicum, is a very old industrial process (acetone-butanol-ethanol-ABE fermentation). The genome of this organism has been sequenced and analysed, leading to important improvements in rational strain construction.

Pathway engineering and synthetic biology using acetogens.

Acetogenic anaerobic bacteria are defined as organisms employing the Wood-Ljungdahl pathway to synthesize acetyl-CoA from CO(2) or CO. Their autotrophic mode of metabolism offers the biotechnological chance to combine use of abundantly available substrates with reduction of greenhouse gases. Several companies have already established pilot and demonstration plants for converting waste gases into ethanol, an important biofuel and a natural product of many acetogens.