RHD zygosity assignments based on most probable genotype and hybrid Rhesus box detection in Tunisia.

Background: Determination of the RHD zygosity is important for genetic counselling and risk evaluation of hemolytic disease of the newborn HDN in women with D iso-immunisation.

Objectives: We proposed to determine the genotype frequencies of the RHD locus using a PCR-SSP method and assignment of the most probable genotype (MPG) and analyse the concordance between the two methods.

Methods: The complete Rh phenotype and the frequencies of RH haplotypes were determined on 506 blood donors.