Increased growth hormone receptor (GHR) degradation due to over-expression of cytokine inducible SH2 domain-containing protein (CIS) as a cause of GH transduction defect (GHTD).

Objective: Children with growth hormone transduction defect (GHTD) have impaired growth and signal transducer and activator of transcription 3 (STAT3) activation. Here, we examine the etiology of GHTD.

Methods: Control (Cf) and GHTD (Pf) children's fibroblasts were induced with hGH, MG132, lactacystin or silence RNA/CIS (siCIS).