Bridging the gap between wellbeing and evaluation: Lessons from IBEST, a French experience.

While evaluators and advocates of alternative indicators both reflect on values and the collective construction of judgements, they do not talk much to each other. The ambition of this paper is to bridge this gap between evaluators and proponents of alternative indicators, by highlighting how an approach dedicated to the construction of alternative indicators can support evaluation practice. To do so, we draw on a participatory project conducted in Grenoble (France), that focused on the construction of indicators of sustainable wellbeing (IBEST).

Spreading of porous vesicles subjected to osmotic shocks: the role of aquaporins.

Aquaporin 0 (AQP0) is a transmembrane protein specific to the eye lens, involved as a water carrier across the lipid membranes. During eye lens maturation, AQP0s are truncated by proteolytic cleavage. We investigate in this work the capability of truncated AQP0 to conduct water across membranes.

Membrane shape modulates transmembrane protein distribution.

Although membrane shape varies greatly throughout the cell, the contribution of membrane curvature to transmembrane protein targeting is unknown because of the numerous sorting mechanisms that take place concurrently in cells. To isolate the effect of membrane shape, we used cell-sized giant unilamellar vesicles (GUVs) containing either the potassium channel KvAP or the water channel AQP0 to form membrane nanotubes with controlled radii. Whereas the AQP0 concentrations in flat and curved membranes were indistinguishable, KvAP was enriched in the tubes, with greater enrichment in more highly curved membranes.

Ab initio and all-atom modeling of detergent organization around Aquaporin-0 based on SAXS data.

A necessary initial step for the application of small angle X-ray scattering (SAXS) as an analytical probe for structural investigations of membrane proteins in solution is the precise knowledge of the structure of spontaneously formed detergent assemblies around the protein. Following our recent article (Berthaud et al. J.

Modeling detergent organization around aquaporin-0 using small-angle X-ray scattering.

Solubilization of integral membrane proteins in aqueous solutions requires the presence of amphiphilic molecules like detergents. The transmembrane region of the proteins is then surrounded by a corona formed by these molecules, ensuring a hydrophilic outer surface. The presence of this corona has strongly hampered structural studies of solubilized membrane proteins by small-angle X-ray scattering (SAXS), a technique frequently used to monitor conformational changes of soluble proteins.

[Gender control and Olympics events from Albertville to Atlanta or good practices in gene biochemistry].

Gender verification in sport was introduced in 1967 based on the insufficient Barr test. For Albertville's Olympic Games we proposed the Medical Commission of IOC to carry out the control for the first time thanks to molecular biochemistry. PCR/SRY amplification is described giving more than 99.