A dimensional approach to maternal attachment state of mind: relations to maternal sensitivity and maternal autonomy support.

The aim of this study was to examine the developmental significance of the newly developed dimensional approach to attachment state of mind by investigating its capacity to predict individual differences in the quality of two caregiving behaviors-maternal sensitivity and maternal autonomy support-that are linked to numerous important child outcomes. Seventy-one upper-middle-class, predominantly French-speaking and Caucasian dyads participated in 3 home visits (34 girls). The Adult Attachment Interview (AAI) was administered when the infants were 8 months old, maternal sensitivity was assessed when they were 12 months old, and maternal autonomy support was assessed at 15 months.

Relations between physiological and cognitive regulatory systems: infant sleep regulation and subsequent executive functioning.

The aim of this report was to investigate the prospective links between infant sleep regulation and subsequent executive functioning (EF). The authors assessed sleep regulation through a parent sleep diary when children were 12 and 18 months old (N = 60). Child EF was assessed at 18 and 26 months of age.

Early Manifestations of Children's Theory of Mind: The Roles of Maternal Mind-Mindedness and Infant Security of Attachment.

This study investigated two aspects of mother-child relationships-mothers' mind-mindedness and infant attachment security-in relation to two early aspects of children's theory of mind development (ToM). Sixty-one mother-child dyads (36 girls) participated in testing phases at 12 (T1), 15 (T2), and 26 months of age (T3), allowing for assessment of maternal mind-mindedness (T1), infant attachment (T2), and child ToM understanding (T3). Results indicated that children's understanding of discrepant desires and visual perspectives was positively related to their mothers' earlier use of appropriate mind-related comments in certain contexts.

From external regulation to self-regulation: early parenting precursors of young children's executive functioning.

In keeping with proposals emphasizing the role of early experience in infant brain development, this study investigated the prospective links between quality of parent-infant interactions and subsequent child executive functioning (EF), including working memory, impulse control, and set shifting. Maternal sensitivity, mind-mindedness and autonomy support were assessed when children were 12 to 15 months old (N = 80). Child EF was assessed at 18 and 26 months.

Initiating a Caregiving Relationship: Pregnancy and Childbirth Factors as Predictors of Maternal Sensitivity.

The aim of this study was to investigate the relations between pregnancy and childbirth factors and subsequent quality of maternal interactive behavior in a sample of 116 full-term infants and their mothers. Mothers reported on the conditions of childbirth when infants were 6-8 months of age, and their interactive behavior was observed during a home visit at 12 months. Results showed that mothers who did not report health problems during pregnancy and who had longer pregnancies, shorter hospital stays, natural deliveries, and infants with greater birthweight were found to be more sensitive during interactions with infants at 12 months.

The phosphate transporter PiT1 (Slc20a1) revealed as a new essential gene for mouse liver development.

Background: PiT1 (or SLC20a1) encodes a widely expressed plasma membrane protein functioning as a high-affinity Na(+)-phosphate (Pi) cotransporter. As such, PiT1 is often considered as a ubiquitous supplier of Pi for cellular needs regardless of the lack of experimental data. Although the importance of PiT1 in mineralizing processes have been demonstrated in vitro in osteoblasts, chondrocytes and vascular smooth muscle cells, in vivo evidence is missing.

An efficient process for the purification of helper-dependent adenoviral vector and removal of helper virus by iodixanol ultracentrifugation.

The preparation of large amount of purified helper-dependent adenoviral vector material is hampered by the lack of development of downstream processes with proven records on separation and recovery efficiencies. In order to facilitate the use of clinical-grade helper-dependent virus material for large-scale in vivo studies, a three-step purification scheme consisting of (1) an anion-exchange chromatography for initial capturing of virus, (2) a shallow iodixanol density gradient ultracentrifugation for the removal of helper virus from helper-dependent virus, and (3) a size-exclusion chromatography for the removal of iodixanol and residual protein contaminants as a polishing step was developed. The use of a fast iodixanol density ultracentrifugation step was highly effective in separating infectious helper-dependent virus from contaminating helper virus.

Maternal and child characteristics as antecedents of maternal mind-mindedness.

This article compares an expanded descriptive measure of maternal mind-mindedness to the original measure. We included assessments of the valence and richness of descriptions given by 106 mothers when asked to talk about their 18-month-old child (55 boys), in addition to assessing the proportion of mind-related descriptors. Contrary to previous findings, the proportion of mental descriptors was not associated with maternal sensitivity.

Intergenerational transmission of attachment in father-child dyads: the case of single parenthood.

Researchers have repeatedly found that intergenerational transmission of attachment is more robust in mother-child dyads than it is in father-child dyads. They have proposed several explanations for the inconsistent father-child findings, including the use of the strange situation procedure, the young age of the children, and the fact that fathers are often less involved in early caregiving than are mothers. The authors aimed to investigate intergenerational transmission in father-child dyads when several conditions are present that may heighten the likelihood of such a transmission.

Attachment-based intervention for maltreating families.

This article presents attachment theory-based intervention strategies as a means of addressing the core parent-child interaction deficits that characterize homes in which children are exposed to maltreatment. The article outlines the socioemotional and cognitive outcomes of maltreatment and proposes that although many prevention programs target different parental and family characteristics, few address the core relationship issues that are at stake. Recent research on attachment-based intervention strategies, aimed at improving the sensitivity and responsiveness of the parenting behaviors that children are exposed to, are presented as providing a means of addressing this domain.

Vascular dysfunction in glycogen storage disease type I.

Objective: To determine cardiovascular disease risk in a larger cohort of patients with glycogen storage disease (GSD) I through the use of noninvasive measures of arterial function and anatomy.

Study Design: Carotid intima media thickness (IMT), radial artery tonometry, and brachial artery reactivity were performed in 28 patients with GSD I (13F/15M, mean age 23 years) and 23 control subjects (19F/4M, mean age 23 years).

Results: The primary outcome measure, mean left distal IMT was greater in the GSD cohort (0.

Polyhexamethylene guanidine hydrochloride-based disinfectant: a novel tool to fight meticillin-resistant Staphylococcus aureus and nosocomial infections.

Polyhexamethylene guanidine hydrochloride (PHMGH), an antimicrobial biocide of the guanidine family, was tested for efficacy against quality-control strains of Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella choleraesuis, meticillin-resistant S. aureus (MRSA) and Escherichia coli. Bactericidal activity against S.

Hyperlipidemia in glycogen storage disease type III: effect of age and metabolic control.

While the presence of hyperlipidaemia in glycogen storage disease (GSD) type Ia and Ib is generally accepted, few investigators have adequately assessed lipid profiles of GSD III in children, in whom the presence of hyperlipidaemia may be most prominent. We analysed the lipid profiles in 44 GSD III patients from 6 months to 30 years of age. Hypertriglyceridaemia and hypercholesterolaemia were common in children younger than 3 years of age.

Rapid and reliable quantification of reovirus type 3 by high performance liquid chromatography during manufacturing of Reolysin.

Reolysin, a human reovirus type 3, is being evaluated in the clinic as an oncolytic therapy for various types of cancer. To facilitate the optimization and scale-up of the current process, a high performance liquid chromatography (HPLC) method has been developed that is rapid, specific and reliable for the quantification of reovirus type 3 particles. Using an anion-exchange column, the intact virus eluted from the contaminants in 9.

A threshold approach to understanding the origins of attachment disorganization.

Disorganized attachment in infancy is known to predict a wide range of maladaptive outcomes, but its origins are poorly understood. Parental lack of resolution concerning loss or trauma has been proposed to result in atypical parenting behaviors, which in turn have a disorganizing effect on the parent-child relationship. The authors review the evidence for this transmission pathway, considering other factors (e.

A new approach for the purification and characterisation of PA49.5, the main prebiotic of Lactococcus lactis subsp. cremoris.

The main autolysin PA49.5, an enzyme that hydrolyzes or destroys the components of a biological endogenous cell or a tissue, was purified 3045 times from the homogenate of a whole cell extract of Lactococcus lactis subsp. cremoris ATCC 9596 (Mc5), with a recovery yield of 52%.

Associations between maternal mind-mindedness and infant attachment security: investigating the mediating role of maternal sensitivity.

In the last 20 years, three meta-analyses suggested that the relation between maternal sensitivity and infant attachment security was lesser in magnitude than originally believed. This led to a search for other parental behaviors likely to contribute to the development of attachment security. Based on previous theoretical propositions and empirical findings suggesting that maternal mind-mindedness may contribute to infant attachment security by favoring maternal sensitivity, the aim of this study was to examine whether sensitivity mediates the relation between maternal mind-mindedness and infant attachment security.

MUC1 initiates Src-CrkL-Rac1/Cdc42-mediated actin cytoskeletal protrusive motility after ligating intercellular adhesion molecule-1.

MUC1, a transmembrane glycoprotein of the mucin family, when aberrantly expressed on breast cancer cells is correlated with increased lymph node metastases. We have previously shown that MUC1 binds intercellular adhesion molecule-1 (ICAM-1) on surrounding accessory cells and facilitates transendothelial migration of MUC1-bearing cells. Nevertheless, the underlying molecular mechanism is still obscure.

Pain Catastrophizing Scale for Francophone Adolescents: a preliminary validation.

Background: In adults, it is well known that high levels of pain catastrophizing are related to increased pain and disability as well as to heightened anxiety and depression. However, due to the lack of a measure of pain catastrophizing adapted for francophone adolescents, little is known about the role of catastrophizing in this population.

Objectives: To adapt the French version of the Pain Catastrophizing Scale (PCS) and to examine the psychometric properties and factorial structure of the PCS for Francophone Adolescents (PCS-Ado).

Development of a suspension serum-free helper-dependent adenovirus production system and assessment of co-infection conditions.

Helper-dependent adenovirus (HDAd), deleted in all viral protein-coding sequences has been designed to reduce immune response and favor long-term expression of therapeutic genes in clinical programs. Its production requires co-infection of E1-complementing cells with helper adenovirus (HAd). Significant progresses have been made in the molecular design of HDAd, but large scale production remains a challenge.

Virus-like particle and viral vector production using the baculovirus expression vector system/insect cell system: adeno-associated virus-based products.

The ability to make a large variety of virus-like particles (VLPs) has been successfully achieved in the baculovirus expression vector system (BEVS)/insect cell system. The production and scale-up of these particles, which are mostly sought as candidate vaccines, are currently being addressed. Furthermore, these VLPs are being investigated as delivery agents for use as therapeutics.

Validation of a high-performance liquid chromatographic assay for the quantification of Reovirus particles type 3.

An anion exchange high-performance liquid chromatography (HPLC) method for the quantification of human Reovirus type 3 particles was validated according to the performance criteria of precision, specificity, linearity of calibration and working range, limits of detection and quantification, accuracy and recovery. Samples taken at various stages of Reovirus purification were used for the validation of the method. The method was specific for Reovirus which eluted around 9.

A simple macroscopic model for the diffusion and adsorption kinetics of r-adenovirus.

The diffusion of viruses toward cells is a limiting step of the infection process. To be modeled correctly, this step must be evaluated in combination with the adsorption of the virus to the cell surface, which is a rapid but reversible step. In this paper, the recombinant adenovirus (rAd) diffusion and its adsorption to 293S cells in suspension were both measured and modeled.

Escherichia coli inactivation mechanism by pressurized CO2.

The effects of pressurized CO2 on the survival of Escherichia coli and the mechanism of cell inactivation were studied. Bacterial cultures were inoculated in nutrient broth and incubated at 30 degrees C for 18 h. Exposure of the cells to CO2 under pressures ranging from 2.