Tentative identification of hemoglobin variants in the Bio-Rad VARIANT II Hb A1C method.

Objective: To evaluate if peak characteristics, the retention time, and percentage of total hemoglobin of an unknown peak on the Bio-Rad VARIANT II Hb A1c method may be used to establish a tentative identification of hemoglobin variants.

Method: The peak characteristics, retention time, and percentage of total hemoglobin obtained on abnormal peaks found on the Bio-Rad VARIANT II Hb A1c method were tabulated and evaluated against the identification of the hemoglobin variant established by the Bio-Rad beta thalassemia HPLC method and hemoglobin electrophoresis at both acid and alkaline pH.

Results: Some hemoglobin variants show specific peak characteristics, retention times, and percentage of total hemoglobin on the Bio-Rad VARIANT II Hb A1c method that allows for tentative identification of the hemoglobin variant.

Comparison of lactate, bilirubin and hemoglobin F concentrations obtained by the ABL 700 series blood gas analyzers with laboratory methods.

Objective: To compare lactate, bilirubin and Hemoglobin F concentrations obtained on ABL 700 series blood gas analyzers with those from laboratory methods.

Design And Method: Pooled neonatal plasma, cord blood and adult plasma samples were used for comparison of bilirubin, hemoglobin F and lactate concentrations respectively.

Results: Results obtained on the ABL 700 series compared favorably (Deming regression slopes 0.