A native phosphoglycolate salvage pathway of the synthetic autotrophic yeast .

Synthetic autotrophs can serve as chassis strains for bioproduction from CO as a feedstock to take measures against the climate crisis. Integration of the Calvin-Benson-Bassham (CBB) cycle into the methylotrophic yeast () enabled it to use CO as the sole carbon source. The key enzyme in this cycle is ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) catalyzing the carboxylation step.

The oxygen-tolerant reductive glycine pathway assimilates methanol, formate and CO in the yeast Komagataella phaffii.

The current climatic change is predominantly driven by excessive anthropogenic CO emissions. As industrial bioprocesses primarily depend on food-competing organic feedstocks or fossil raw materials, CO co-assimilation or the use of CO-derived methanol or formate as carbon sources are considered pathbreaking contributions to solving this global problem. The number of industrially-relevant microorganisms that can use these two carbon sources is limited, and even fewer can concurrently co-assimilate CO.

Tailored extraction and ion mobility-mass spectrometry enables isotopologue analysis of tetrahydrofolate vitamers.

Climate change directs the focus in biotechnology increasingly on one-carbon metabolism for fixation of CO and CO-derived chemicals (e.g. methanol, formate) to reduce our reliance on both fossil and food-competing carbon sources.

Conversion of CO into organic acids by engineered autotrophic yeast.

The increase of CO emissions due to human activity is one of the preeminent reasons for the present climate crisis. In addition, considering the increasing demand for renewable resources, the upcycling of CO as a feedstock gains an extensive importance to establish CO-neutral or CO-negative industrial processes independent of agricultural resources. Here we assess whether synthetic autotrophic () can be used as a platform for value-added chemicals using CO as a feedstock by integrating the heterologous genes for lactic and itaconic acid synthesis.

Development, characterization, and application of a 2-Compartment system to investigate the impact of pH inhomogeneities in large-scale CHO-based processes.

Large-scale bioreactors for the production of monoclonal antibodies reach volumes of up to 25 000 L. With increasing bioreactor size, mixing is however affected negatively, resulting in the formation of gradients throughout the reactor. These gradients can adversely affect process performance at large scale.

Simultaneous non-polar and polar lipid analysis by on-line combination of HILIC, RP and high resolution MS.

Given the chemical diversity of lipids and their biological relevance, suitable methods for lipid profiling and quantification are demanded to reduce sample complexity and analysis times. In this work, we present a novel on-line chromatographic method coupling hydrophilic interaction liquid chromatography (HILIC) dedicated to class-specific separation of polar lipid to reversed-phase chromatography (RP) for non-polar lipid analysis. More specifically, the void volume of the HILIC separation-consisting of non-polar lipids- is transferred to the orthogonal RP column enabling the on-line combination of HILIC with RP without any dilution in the second dimension.