Publications by authors named "Bernadette Hym"
Background: The precision of the formulae used to estimate glomerular filtration rate (GFR) decreases when the serum creatinine (SCr) assay is biased compared with the assay used during the development of the formulae.
Methods: For 100 children referred for 51Cr-EDTA clearance (CLEDTA), SCr was measured with a JAFFE (classic Jaffe colorimetric creatinine assay), a compensated Jaffe (COMP), an enzymatic (ENZ) and an HPLC assay. A population pharmacokinetics approach based on a non-linear mixed effects model (NONMEM) was used to model the relationships between the CLEDTA and physiopathological/analytical variables.
Article Synopsis
- The study aimed to evaluate the variability and accuracy of different serum creatinine measurement methods, focusing on how standardized calibration affects this variability.
- 30 human serum samples and 3 reference materials were tested using 17 different creatinine assays, comparing results from manufacturers’ calibration methods to two standardized calibration approaches.
- Although the calibration method influenced variability, none of the standardized procedures significantly reduced overall inter-assay variability, confirming the need for caution in using creatinine assays for estimating kidney function.