Fluorescence in situ hybridization study of TEL/AML1 fusion and other abnormalities involving TEL and AML1 genes. Correlation with cytogenetic findings and prognostic value in children with acute lymphocytic leukemia.

Background And Objectives: The TEL/AML1 fusion is the most common genetic abnormality found in childhood acute lymphoblastic leukemias (ALL). Although it is very difficult to identify by conventional cytogenetic techniques it can be readily detected using fluorescence in situ hybridization (FISH). We carried out cytogenetic and FISH studies on 42 children with ALL in order to know the frequency of this translocation in our population, the incidence of TEL and/or AML1 gene alterations, and their correlation with clinical evolution and prognosis.

Flow cytometry and the study of central nervous disease in patients with acute leukaemia.

Central nervous system (CNS) leukaemia is still a matter of debate and new technologies are required to improve the classic morphological definition. One hundred and sixty-eight cerebrospinal fluid (CSF) samples from 31 patients with acute leukaemia were analysed by flow cytometry and conventional cytology. Concordant positive and negative findings were found in 158 samples but 10 produced discrepant results.

Chronic recurrent multifocal osteomyelitis after acute lymphoblastic leukaemia.

We describe an 8 year old girl who developed chronic recurrent multifocal osteomyelitis (CRMO) in the ilium and clavicle. Treatment for an acute lymphoblastic leukaemia had been finished two months before. After antibiotic therapy, the clinical symptoms improved and no fresh lesions appeared.

Infection caused by the nonfermentative gram-negative bacillus CDC group IV c-2: case report and literature review.

A 10-year-old girl with acute lymphocytic leukemia developed nosocomial septicemia caused by the gram-negative bacterium CDC group IV c-2. Recovery of the patient followed appropriate treatment with ceftriaxone, to which the organism was susceptible in vitro. Four other reported cases of infection caused by this organism are reviewed.

[Capnocytophaga spp. bacteremia in compromised patients].

Background: To analyze the clinical characteristics of three patients with Capnocytophaga spp. bacteremia.

Methods: We have review the clinical charts of three patients with Capnocytophaga spp.

[Acute lymphoblastic leukemias off therapy: course of 80 cases after treatment cessation].

Purpose: To retrospectively analyse all the patients diagnosed and treated in the same hospital for acute lymphoblastic leukaemia (ALL) in whom therapy was suppressed after sustained complete remission (CR) for a variable period.

Patients And Methods: Eighty cases of ALL treated at the Jiménez Díaz Foundation between 1968 and 1991 were revised. Treatment had been suppressed after 60 months of maintained CR (1968-1974) or after 28 months (1986-1991), with a median follow-up after suppression of 6 years.

Immunophenotypic and Gene Rearrangement Analysis in Null- or T-Cell Neoplasias: Study of 16 Cases.

Immunophenotypic and molecular studies were performed in sixteen cases of T-cell lymphoproliferative disorders. These included eleven patients with peripheral T-cell lymphoma, two thymic lymphomas and three patients with T-gamma lymphocytosis. Peripheral T-cell lymphomas were of both low and high grades.

[Acute promyelocytic leukemias: clinico-biological aspects, prognostic factors, therapeutic response, and possibilities of cure in 34 cases (1970-1988)].

Thirty-four new cases of acute promyelocytic leukaemia (M3) were diagnosed at the authors' Centre between 1970 and 1988 (19 males and 15 females) with ages between 5 and 73 years (median age, 32 years). Three cases were of the hypogranular variant or M3-v (8.8%).

[Open lung biopsy in immunocompromised patients with diffuse pulmonary infiltrates].

In order to evaluate the diagnostic usefulness of open lung biopsy and to determine how could it influence the treatment and the evolution of the disease, the clinical histories of 19 immunocompromised patients with diffuse lung infiltrates were reviewed. One or more specific diagnosis were obtained in 14 patients (73%) by open lung biopsy. However, the initial treatment was modified, in view of the results of the biopsy only in 3 cases (15%).

[Post-hepatitis non-A non-B transient medullar aplasia and acute lymphoblastic leukemia].

We report a clinical hematologic sequential process consisting of transient marrow aplasia post-hepatitis (non A-non B) preceding acute lymphoblastic leukemia. There have not been other reports mentioning a similar evolution. Authors outline the possible pathogenetic mechanisms involved in the sequence of events described.

[Familial hemolytic-uremic syndrome associated with Coxsackie B infection].

Authors describe hemolytic uremic syndrome (HUS) in two siblings whose onset occurred within eight days. There was evidence of having recent infection with Coxsackie B virus. Clinical course, treatment and outcome of patients are reported, showing differences on the severity of renal failure and platelet consumption.