Use of a Monoclonal Antibody and Two Enzyme-Linked Immunosorbent Assay Formats for Detection and Quantification of the Substitution of Caprine Milk for Ovine Milk.

A stable hybridoma cell line (B2B) has been produced that secretes a monoclonal antibody (MAb) specific for goat's milk αcasein. The MAb B2B was used in two enzyme-linked immunosorbent assay (ELISA) formats for the detection and quantification of the presence of goat's milk in ewe's milk. In the indirect ELISA format the limit of detection was 0.

Immunostick Colorimetric Assay for Detection of Pseudomonas spp. in Refrigerated Meat and Milk.

Monoclonal antibodies generated against live cells of Pseudomonas fluorescens have been used in an immunostick enzyme-linked immunosorbent assay (ELISA) format for the detection of Pseudomonas spp. in refrigerated meat and milk. The detection threshold for the immunostick ELISA assay developed in this work is 10 CPU cm for meat and 10 CPU ml for milk samples.

Development of an Enzyme-Linked Immunosorbent Assay for the Identification of Smoked Salmon ( Salmo salar ),Trout ( Oncorhynchus mykiss ) and Bream ( Brama raii ).

An indirect enzyme-linked immunosorbent assay (ELISA) has been developed for the identification of smoked meat from salmon ( Salmo salar ), trout ( Oncorhynchus mykiss ), and bream ( Erama raii ). The assay uses polyclonal antibodies raised in rabbits against soluble proteins of muscle from salmon (anti-SSP), trout (anti-TSP), and bream (anti-BSP) which are rendered species-specific by blocking them with the heterologous soluble muscle proteins. The blocked antibodies were used to detect the samples from smoked fish bound to the wells of a microtiter plate.

Immunostick ELISA for Detection of Cow's Milk in Ewe's Milk and Cheese Using a Monoclonal Antibody against Bovine β-Casein.

An immunostick enzyme-linked immunosorbent assay (ELISA) has been developed for the rapid detection of cow's milk in ewe's milk or cheese. The assay uses a monoclonal antibody (AH4) produced against bovine β-casein for the detection of cow's milk or cheese bound to the paddles of immunostick tubes. This immunostick ELISA allows the visual identification of ewe's milk containing more than 1% of cow's milk or cheese samples containing more than 0.

Detection of Bovine Milk in Ovine Milk by a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA).

A sandwich ELISA (enzyme-linked immunosorbent assay) was developed for the detection of defined amounts of bovine milk (1-30%) in ovine milk. Polyclonal antibodies were raised in rabbits against bovine whey proteins (BWP). Resultant antibodies were affinity purified by immunoadsorption of the crude antiserum onto columns containing immobilized ovine, caprine, and BWP, followed by elution of the bovine milk specific antibodies (anti-BWP) from the column containing the bovine proteins.

Sandwich ELISA for Detection of Pig Meat in Raw Beef Using Antisera to Muscle Soluble Proteins.

A double-antibody sandwich ELISA (enzyme-linked immunosorbent assay) has been successfully developed for the detection of defined amounts of pig meat (1-50%) in raw beef. Antibodies against pig sarcoplasmic extracts were produced in rabbits. Pig-specific antibodies were affinity purified by removing antibodies which crossreacted with horse, chicken or beef extracts followed by immunoadsorption and elution from a pig-extract column.

Effect of Carbon Dioxide and Oxygen Enriched Atmospheres on the Shelf-Life of Refrigerated Pork Packed in Plastic Bags.

Changes in bacterial numbers, metmyoglobin percentage and 2-thiobarbituric acid number during the chill storage of pork longissimus dorsi packed with air, carbon dioxide, carbon dioxide and oxygen or vacuum-packed in plastic bags of high (polyethylene) and low (Cryovac BB-1) permeability to gases were studied. The fast increase of viable counts in polyethylene bags showed that plastic films of high permeability are not suitable to extend the shelf-life of meat using modified atmospheres. In Cryovac bags both carbon dioxide-enriched atmospheres and vacuum-packaging inhibited aerobic organisms, keeping the bacterial flora (mainly lactobacilli and Brochothrix thermosphacta ) below the level of 10/cm for about 3 weeks.