Publications by authors named "Bergisa Hildebrand"
Purification and analysis of a 5kDa component of enamel matrix derivative.
J Chromatogr B Analyt Technol Biomed Life Sci
October 2007
Article Synopsis
- The study utilized high performance liquid chromatography (HPLC) to analyze a 5kDa component from enamel matrix derivative (EMD), which is used in periodontal regeneration.
- Initial purification was done with size-exclusion chromatography (SEC), and subsequent analysis revealed four distinct peaks of the 5kDa component through reversed-phase HPLC (RP HPLC).
- From an initial 1200 mg of EMD, about 65 mg of the 5kDa component was obtained, achieving a 60% recovery rate, with SEC being suitable for qualitative and RP HPLC suitable for both qualitative and quantitative analysis.