[Low-manifest infections with CNS damage in patients in prolonged unconscious state of non-inflammatory etiology].

Aim: Study of specter of low-manifest infections (LMI) with central nervous system (CNS) damage and their role in patients in prolonged unconscious state (PUS) of noninflammatory etiology.

Materials And Methods: 32 patients (23 male, 9 female; age 14-58) in PUS of various etiology were examined. The main group (18 patients) received therapy against all infectious diseases including LMI; control group (14 patients)--only against common and nosocomial microflora.

[Development and use of test systems for the evaluation of sensitivity of Trichomonas vaginalisto preparations of the 5-nitroimidazole and 5-nitrofuran groups].

Aim: Development of test system for the evaluation of sensitivity of Trichomonas vaginalis to preparations of the 5-nitroimidazole and 5-nitrofuran groups.

Materials And Methods: Determination of minimal cidic concentration (MCC) of antiprotozoal preparations was carried out by cultivating laboratory T. vaginalis strains in wells of plates with nutrient medium containing varying concentrations of these preparations.

[Choice of probiotic for rational therapy of infection caused by Klebsiella in children].

Aim: To choose lactobacilli-contained probiotic for complex treatment of acute enteric infection caused by Klebsiella in infants.

Materials And Methods: On the basis of bacteriological analysis the group consisting of 40 infants with acute enteric infection caused by Klebsiella was formed. Efficacy of three probiotic preparations--lactobacterin, vitaflor, and biobacton--was assessed depending on biological features of causative agents and contents of lactobacilli and bifidobacteria in obligate gut microflora.

Morphofunctional changes in guinea pig ovaries in experimental chronic herpesvirus infection.

The ovaries of adult guinea pigs infected with herpesvirus-2 were examined by light microscopy under conditions of latent infection and relapse of infectious process. Morphofunctional changes in the ovarian follicles at all stages of folliculogenesis and in ovarian stroma were caused by the negative effects of type 2 herpes simplex virus.

[Biochemical mechanisms of realization of antiviral and interferon-inducing activity of amixine and its analogs].

Antiviral and interferon inducing activity of the amixine and its some derivatives, as well as their influence on the proteolytic enzymes activity, monooxygenase activity of the microsomal fraction, level of the lipids peroxidation were studied. Lack of correlation between antiviral and interferon inducing activity in the investigated series of compounds was found. Vice versa, the good correlation between interferon inducing activity and the elastase-like activities inhibition ability of the compounds was observed.

[The adhesive activity of Klebsiella pneumoniae controlled by plasmid 55 MD].

In K. pneumoniae clinical strains a nonconjugative plasmid, denoted as pAdh-55 and responsible for their adhesive activity with respect to HEp-2 cells, was detected. The comparison of genetically related pairs of K.

[The hemodynamic and laboratory biochemical characteristics of the nephropathy in convalescent patients after hemorrhagic fever with renal syndrome].

Laboratory and functional tests were made in 50 convalescents who had suffered hemorrhagic fever with renal syndrome (HFRS). It is shown that nephropathy in such patients runs with a decline in renal functional reserve indicative of intraglomerular hypertension, impaired ability of the kidneys for osmotic urine concentration, defective tubular reabsorption of beta 2-microglobulin, microcirculatory disorders, collagen disbolism, high levels of uric acid in the blood. The occurrence of hyperuricemia and intraglomerular hypertension in HFRS convalescents calls for special consideration as leading nonimmune factors of nephropathy progression.

Genetic and molecular R-plasmid analysis of Enterobacteriaceae hospital strains at Children's Hospitals of the former USSR.

R-plasmids from Enterobacteriaceae clinical strains, mainly Klebsiella and Serratia, isolated at different neonatal and children's hospitals of different cities of the former USSR for 10 years, were studied for their possible influence on the bacterial host phenotype. Hospital R-plasmids of stable inheritance persisted in hospitals from 2 to 7 years and were disseminated among strains of different genera (Klebsiella, Serratia, Enterobacter) and among different units. The data showed a possibility of long-term molecular rearrangements of R-plasmids in the hospital settings and an acquisition of genetic determinants encoding enterotoxin production.

[The incidence of finding and characteristics of Klebsiella pneumoniae strains isolated in neonatal infections].

The clinical and laboratory study of 190 hospitalized children revealed that in 122 cases the course of the underlying disease was complicated by Klebsiella infection. From different pathological material obtained from 122 patients 158 K. pneumoniae cultures were isolated.

[The resistance plasmid of Klebsiella pneumoniae that controls its cytotoxic activity].

R-plasmid (40 MD) isolated from K. pneumoniae hospital strain makes Escherichia coli strain J62 capable of inducing a cytotoxic effect which can be detected in Hep-2 cell culture. In contrast to the initial E.

[A comparative analysis of the polypeptides of the the Sindbis and Karelian fever viruses].

In pulse-chase experiments with Karelian fever virus-infected cells, proteins were found with molecular weights of 130, 98, 78, and 62 kD of which the first, second and fourth were classified as polypeptide precursors of the structural proteins of virion. The molecular weights of proteins E1, E2 and C of 52, 47 and 34 kD, respectively, as well as isoelectric points of isolated glycoproteins (pI E1 = 6.3, pI E2 = 8.

[Antiviral activity of ribamidyl in experimental infections with California encephalitis group viruses].

A preparation of ribamydil, an analogue of natural nucleosides, synthesized at the Latvian SSR Institute of Organic Chemistry showed a sufficiently high activity against bunyaviruses of California encephalitis complex both in vitro and in vivo. Various modifications of the enzyme immunoassay may be used for control of the effectiveness of treatment with this drug. Some advantages of the subcutaneous route over the intramuscular one were found.

[Level of the C3-component of complement in the blood serum of sick children].

The levels of the complement C3 component have been measured in children with various diseases (immunodeficiency states, food allergy, rheumatoid arthritis, severe and medium-severe forms of acute respiratory viral infections), and the complement consumption in the course of alternative activation has been examined. The findings evidence a relationship between the complement C3 component consumption and the blood serum IgA level, as well as between the C3 level and the neutrophilic phagocytic and metabolic activities.

[Antiviral and immunostimulating effect of maleic anhydride copolymers in experimental neuroviral infections].

The virus-inhibiting and immunostimulating activity of Soviet preparations, maleic anhydride copolymers, was demonstrated in alpha-, flavi-, and bunyavirus infections. Positive results were obtained in subcutaneous and intraperitoneal inoculations of the preparations used in prophylactic and therapeutic-prophylactic schedules. Stimulation of vaccination immunity was observed after combined use of copolymers and the vaccine against Eastern equine encephalomyelitis.

[Isolation of the Tahyña bunyavirus in the Arctic].

Tahyna virus (Bunyaviridae, Bunyavirus, the California encephalitis complex) was isolated from Aedes communis complex mosquitoes collected at the border of the north-taiga landscape zone (in latitude 68 degrees North and longitude 33 degrees East) at the Kolsky peninsula (the Murmansk region). The LEIV-9843 Mur strain was isolated from 2.4 thousand mosquitoes collected there (altogether 3.

[Isolation of the causative agent of Karelian fever from Aedes sp. mosquitoes].

The results of isolation of an Alphavirus strain from Aedes sp. mosquitoes collected in the focus of Karelian fever are presented. According to the results of serological studies, the isolated strain LEIV-9298 Karelia belongs to the antigenic complex Sindbis-Western equine encephalomyelitis.

[Sakhalin antigenic group (Bunyaviridae family)].

Serological study of viruses belonging to the Sakhalin group showed Paramushir virus isolated in the USSR and Avalon virus isolated in Canada to be identical in all the tests used. The data on the cultural properties of the Sakhalin group viruses, the virions buoyant densities in sucrose gradient, the data on thermal sensitivity and UV inactivation of the viruses, on their morphology and morphogenesis are presented.