Single cell spatial transcriptomics reveals distinct patterns of dysregulation in non-neuronal and neuronal cells induced by the Alzheimer's risk gene mutation.

Introduction: The R47H missense mutation of the TREM2 gene is a strong risk factor for development of Alzheimer's Disease. We investigate cell-type-specific spatial transcriptomic changes induced by the mutation to determine the impacts of this mutation on transcriptional dysregulation.

Methods: We profiled 15 mouse brain sections consisting of wild-type, , 5xFAD and ; 5xFAD genotypes using MERFISH spatial transcriptomics.