Surface heparinization and blood compatibility modification of small intestinal submucosa (SIS) for small-caliber vascular regeneration.

Objectives: This study aims to investigate the small intestinal submucosal (SIS) surface after heparinization with the hypothermia plasma technique, to improve the blood compatibility of SIS, and to explore the possibility of construction of small-caliber vascular grafts with modified SIS scaffolds in vivo.

Methods: SIS films prepared from jejunums of pigs were processed for surface treatment at different time periods with the argon plasma initiation technique under vacuum, and were then immediately immersed in 4% (m/v) heparin sodium solution for 24-h heparinization. The surface morphologies of heparinized SIS were observed under a scanning electron microscope (SEM).

[Effects of different stress environments on growth of tissue engineering blood vessels].

Objective: To explore the influence of different stress environments on the growth of tissue engineering blood vessels in vivo.

Methods: The engineering vascular scaffolds were prepared with the porcine small intestinal submucosa(SIS) wrapping vascular endothelial cells and smooth muscle cells,which were implanted into the subcutaneous tissue (subcutaneous group), the femoral quadriceps (intramuscular group), and sheathed the femoral artery (perivascular group) respectively. Four weeks postoperatively, these cultured tissues were harvested, and evaluated by macroscopic observation and histology detection.

[Arthroscopically assisted treatment of tibial plateau fractures by tractive reduction with external fixator].

Objective: To study the clinical effect of tractive reduction with external fixator and arthroscopically assisted treatment for tibial plateau fractures.

Methods: From February 2003 to January 2005, a total of 26 cases with tibial plateau fractures were reviewed. There were 4 cases of type I fracture, 5 type II, 4 type III, 6 type IV, 5 type V and 2 type VI based on Schatzker criteria.

[Improvement of blood compatibility of small intestinal submucosa used as engineering vascular scaffolds by nano-bionic surface modification].

Objective: To investigate the effects of nano-bionic surface modification in improving the blood compatibility of small intestinal submucosa (SIS) used as engineering vascular scaffolds.

Methods: SIS films were obtained from pig and underwent nano-bionic surface modification with plasma initiation technique. Scanning electron microscopy (SEM) was used to observe the morphological features, including water contact angle, of the SIS films after nano-bionic surface modification.