This research aimed to systematically identify and preliminarily validate the expressed sequence tag (EST) information using Simple Sequence Repeat (SSR) and provide evidence for further development of SSR molecular marker. The definition of general SSR features of EST splicing sequences and development of SSR primers founded the basis of diversity analysis and variety identification for tree resource. 1134 SSR loci were identified in the EST splicing sequence and distributed in 840 Unigene.
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