Publications by authors named "Benjamin T Shepperd"
Combining ion pairing agents for enhanced analysis of oligonucleotide therapeutics by reversed phase-ion pairing ultra performance liquid chromatography (UPLC).
J Chromatogr B Analyt Technol Biomed Life Sci
June 2011
Article Synopsis
- The field of oligonucleotide therapeutics utilizes synthetic RNA and DNA to develop new treatments, driven by advancements in understanding their mechanisms and design.
- Significant interest in these therapeutics arises from the quicker development of lead molecules and their potential to address tough-to-treat diseases.
- A major challenge is creating effective methods for analyzing the purity of these compounds, as conventional techniques struggle with closely related impurities, but research shows that using multiple ion pairing agents can improve analysis and resolution.
A forced degradation study of a proprietary short interfering RNA (siRNA) molecule most of whose constituent nucleotides have been modified at the 2' position was conducted to assess degradation pathways and stability liabilities. The siRNA was subjected to various conditions as a solid and in solution followed by analysis with reverse-phase ultra-performance liquid chromatography-mass spectrometry. Positional isomers of degradants gave rise to multiple chromatographic peaks with identical masses.
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