Profiling of the Klebsiella pneumoniae Phosphoproteome under Iron-Limited and Iron-Replete Conditions.

Klebsiella pneumoniae was compared across iron-limited and iron-replete conditions to assess changes within the phosphoproteome using quantitative mass spectrometry. These comparative proteomic data provide insights into cellular responses to nutrient limitation and how nutrient requirements may be exploited to provide potential antimicrobial targets.

Phosphoproteome Profiling of Klebsiella pneumoniae under Zinc-Limited and Zinc-Replete Conditions.

The bacterial pathogen Klebsiella pneumoniae causes nosocomial infections with the acquisition of multidrug resistance, impeding treatment options. This study investigated the effect of zinc limitation on the phosphoproteome of K. pneumoniae using quantitative mass spectrometry.

Resolving the Temporal Splenic Proteome during Fungal Infection for Discovery of Putative Dual Perspective Biomarker Signatures.

Fungal pathogens are emerging threats to global health with the rise of incidence associated with climate change and increased geographical distribution; factors also influencing host susceptibility to infection. Accurate detection and diagnosis of fungal infections is paramount to offer rapid and effective therapeutic options. For improved diagnostics, the discovery and development of protein biomarkers presents a promising avenue; however, this approach requires knowledge of infection hallmarks.

Examining the Impacts of CO Concentration and Genetic Compatibility on Perennial Ryegrass- var Interactions.

Perennial ryegrass () is the most cultivated cool-season grass worldwide with crucial roles in carbon fixation, turfgrass applications, and fodder for livestock. forms a mutualism with the strictly vertically transmitted fungal endophyte, var . The fungus produces alkaloids that protect the grass from herbivory, as well as conferring protection from drought and nutrient stress.

Iron Limitation in Defines New Roles for Lon Protease in Homeostasis and Degradation by Quantitative Proteomics.

Nutrient adaptation is key in limiting environments for the promotion of microbial growth and survival. In microbial systems, iron is an essential component for many cellular processes, and bioavailability varies greatly among different conditions. In the bacterium, , the impact of iron limitation is known to alter transcriptional expression of iron-acquisition pathways and influence secretion of iron-binding siderophores, however, a comprehensive view of iron limitation at the protein level remains to be defined.

Decoding communication patterns of the innate immune system by quantitative proteomics.

The innate immune system is a collective network of cell types involved in cell recruitment and activation using a robust and refined communication system. Engagement of receptor-mediated intracellular signaling initiates communication cascades by conveying information about the host cell status to surrounding cells for surveillance and protection. Comprehensive profiling of innate immune cells is challenging due to low cell numbers, high dynamic range of the cellular proteome, low abundance of secreted proteins, and the release of degradative enzymes (e.