Publications by authors named "Benjamin D Huckle"
Article Synopsis
- - Pseudomonas strain NCIMB10586 is capable of producing the antibiotic mupirocin and shows promise for industrial applications, with genomic studies underway to understand its genetics and physiology better.
- - Researchers improved the quality of the genome sequence by combining various sequencing methods, fixing numerous errors that affected key coding regions, and developed a detailed annotation pipeline.
- - RNA sequencing was conducted over 18 hours to analyze gene expression, revealing that mupirocin production peaks later in growth, while the resistance gene is consistently expressed throughout the growth period.
is an industrially important actinomycete whose genetic manipulation is limited by low transformation and conjugation efficiencies, low levels of recombination of introduced DNA, and difficulty in obtaining consistent sporulation. We describe the construction and application of versatile vectors for Cas9-mediated genome editing of this strain. To design spacer sequences with confidence, we derived a highly accurate genome assembly for an isolate of the type strain (ATCC 27064).
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