Publications by authors named "Benjamin D Dratch"
Article Synopsis
- The study focuses on how IgM antibodies, which are produced in patients with hemophilia A, interact with factor VIII (FVIII) and potentially influence the development of harmful IgG inhibitors.
- Researchers identified specific FVIII-targeting IgM antibodies from mice and analyzed their binding capabilities using advanced lab techniques.
- Results indicated that while the IgM antibodies can bind to FVIII, they do not significantly influence the production of IgG inhibitors, suggesting that IgM's role may be limited in this context.
Nitration reactions are crucial for many industrial syntheses; however, current protocols lack site specificity and employ hazardous chemicals. The noncanonical cytochrome P450 enzymes RufO and TxtE catalyze the only known direct aromatic nitration reactions in nature, making them attractive model systems for the development of analogous biocatalytic and/or biomimetic reactions that proceed under mild conditions. While the associated mechanism has been well-characterized in TxtE, much less is known about RufO.
View Article and Find Full Text PDFThis study utilizes the FMN-dependent NADH:quinone oxidoreductase from PAO1 to investigate the effect of introducing an active site negative charge on the flavin absorption spectrum both in the absence and presence of a long-range electrostatic potential coming from solution ions. There were no observed changes in the flavin UV-visible spectrum when an active site tyrosine (Y277) becomes deprotonated . These results could only be reproduced computationally using average solvent electrostatic configuration (ASEC) QM/MM simulations that include both positive and negative solution ions.
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