Elastomeric and Conductive Nerve Conduits From Poly(Glycerol-Sebacate)/Carbon Nanofibers (PGS/CNFs).

Many patients suffer from peripheral nerve injury, which can impair their quality of life. Restoring nerve tissue is difficult due to the low ability of nerves to regenerate. Nerve conduits are designed to help peripheral nerve regeneration by providing a scaffold that can match the tissue characteristics, facilitate cellular activities, and be easily implanted.

Preparation of hybrid meniscal constructs using hydrogels and acellular matrices.

To search for a suitable meniscus repair material, acellular hybrid scaffolds consisting of cross-linkable 3-D interpenetrating network structures were obtained by decellularization of the meniscus tissues followed by integration of the gel system. Decellularization efficiency was confirmed using a DNA quantification assay (82% decrease in DNA content) and histological stainings. In the second part of the study, the gelatin molecule was functionalized by adding methacrylic anhydride and the degree of functionalization was found to be 75% by (Proton-Nuclear Magnetic Resonance) H-NMR.

Surface channel patterned and endothelialized poly(glycerol sebacate) based elastomers.

Prevascularization of tissue equivalents is critical for fulfilling the need for sufficient vascular organization for nutrient and gas transport. Hence, endothelial cell culture on biomaterials is of great importance for researchers. Numerous alternate strategies have been suggested in this sense, with cell-based methods being the most commonly employed.

Preparation of decellularized optic nerve grafts.

Background: Decellularized tissues based on well-conserved extracellular matrices (ECMs) are a common area of research in tissue engineering. Although several decellularization protocols have been suggested for several types of tissues, studies on the optic nerve have been limited.

Methods: We report decellularization protocol with different detergent for the preparation of acellular optic nerve and tissues were examined.

Use of supercritical CO in soft tissue decellularization.

Supercritical carbon dioxide (scCO) is being used as an alternative approach to the traditional methods for the decellularization of tissues. This chapter describes the use of scCO for the decellularization of optic nerve, myocardium, and cornea tissues. The main goal of this method is to burst the cells with high-pressure, remove them from the tissues and to maintain the extracellular matrix structure of the native tissues.