[Role of helminth antigens in the abnormal mitosis of bone marrow cells in laboratory animals].

The intraabdominal administration of somatic extracts of the cestodes Hydatigera taeniaformis Batsch 1786, Lamarck, 1816 and Diphyllobothrium latum Linnaeus, 1758 and the nematodes Anisakis simplex larva Rudolphi 1809, Toxocara canis Railliet et Henry, 1912 in albino mice proved that these helminths had a karyopathic effect on the bone marrow cells of the animals. The antigenic composition of these extracts was investigated using the agar gel immunodiffusion test. The antigenic composition of the parasites was ascertained to affect their karyopathic properties.

[Design of a recombinant antigen for the diagnosis and prevention of larval hydatidosis].

The paper deals with the design of recombinant vector make-ups, with the expression of Echinococcus hybrid proteins, and with the study of their immunogenic properties. Theoretical rationale is given for the choice of the parasitic gene superexpression system (E. coli cells of SG 13009 strain--recombinant plasmid pQE/EgF).

[Echinococcus granulosus genotyping by PCR-RFLP].

A method to assess the length polymorphism of restriction fragments of polymerase chain reaction (PCR-LPRF) has been developed and used for the genomic typing of E. granulosus. A scheme optimal under our conditions has been chosen for analysis.

[Laboratory diagnosis of toxoplasmosis using polymerase chain reaction].

Polymorphism of clinical manifestations in Toxoplasma infection and variegated disease patterns virtually rule out the diagnosis based solely on clinical symptoms, which makes modern laboratory tests particularly important. Amplification test system based on the polymerase chain reaction has been developed for the diagnosis of Toxoplasma infection. Computer analysis of nucleotide sequence of Toxoplasma gondii surface antigen gene P30 was analyzed, which helped choose and synthesize specific oligonucleotide primers.

[Use of polymerase chain reaction for identifying helminth DNA from the species Trichinella, Fasciola, Echinococcus, Nematodirus, Taenia].

We have used the polymerase chain reaction (PCR) and random amplified polymorphic DNA (RAPD) method to identify DNA polymorphism in three classes of helminths, Cestoda, Nematoda and Trematoda. In this study, RAPD markers have been used for a description of the genetic variability between the families, genera, species and isolates of helminths. We optimized the conditions of the RAPD analysis and revealed the genetic variability of helminths belonging to different taxonomic groups.

[Phasic changes in the functional activity of alpha2- and beta2- subtypes of platelet adrenergic receptors--objective criteria of adrenoreactive imbalance in the evaluation of severity of nephropathies in pregnancy].

The pregnant suffering from nephropathies were found to have autonomic nervous control disorders closely related to the major platelet vascular and plasma hemostatic, hemodynamic, and microcirculatory parameters. A combined examination of the macro- and ++micro-hemodynamics and status of adrenoreactive structures assessed the adaptation of a pregnant to nephropathy and her reserve potentialities. Adrenoreactive structure derangement is a measure of the severity of nephropathy.

[Gonadotropic and sex hormones in women with systemic lupus erythematosus].

The authors studied gonadotropic and sex hormones of the blood as well as estrogens and hestagens in 100 women (70--with systemic lupus erythematosus and 30 healthy subjects). A decrease in the level of folliculostimulating hormone has been observed. Hyperestrogenic nature of the hormonal balance was diagnosed in patients with the newly developed systemic lupus erythematosus.

[Mitochondrial respiratory activity of the trematode Fasciola hepatica].

Polarographic studies have been made on the respiratory activity of isolated mitochondria of the trematode F. hepatica. Respiratory chain transferring electrons to oxygen and which is sensitive to cyanide was found in the mitochondria.

[Hormone and lipid interrelations in young women with normal and disturbed balance of sex steroids].

Altogether 121 patients with sclerocystic ovaries and 40 normal women aged 16 to 35 years were examined for excretion of estrogens, androgens and pregnandiol with urine and for the blood content of total cholesterol (TC), triglycerides (TG) and high density lipoprotein cholesterol (HDLC) followed by hyperlipoproteinemia (HLP) phenotyping. In the group of normal women, low production of estrogens and pregnandiol was coupled with a reduction in the content of HDLC and an increase in that of TG. In the patients, no relationship was established between the changes in the concentration of estrogens and pregnandiol and the lipid content.

[Cardiovascular disorders, autonomic disorders and atherogenic dyslipoproteinemias in young women with the sclerotic ovary syndrome].

The authors studied 158 young women with the syndrome of sclerocystic ovaries (SCO) and 40 healthy subjects. The patients showed a high rate of vegetovascular dystonia, predominantly according to the hyperparasympathicotonic type, cardialgia and dyspnea. In 54.

[Enzymatic hydrolysis of fat in the presence of Lamblia duodenalis (in vitro)].

The influence of Lamblia on the hydrolysis of fat by lipase was studied in vitro. The hydrolysis rate of fat in the presence of live Lamblia and without them was determined colorimetrically by the quantity of the formed glycerine. In addition, the kinetics of this reaction was studied by the method of compensating potentiometry by neutralization of fat acids with alkali.