Nonlinear microscopy using impulsive stimulated Brillouin scattering for high-speed elastography.

The impulsive stimulated Brillouin microscopy promises fast, non-contact measurements of the elastic properties of biological samples. The used pump-probe approach employs an ultra-short pulse laser and a cw laser to generate Brillouin signals. Modeling of the microscopy technique has already been carried out partially, but not for biomedical applications.

Recent progress and current opinions in Brillouin microscopy for life science applications.

Many important biological functions and processes are reflected in cell and tissue mechanical properties such as elasticity and viscosity. However, current techniques used for measuring these properties have major limitations, such as that they can often not measure inside intact cells and/or require physical contact-which cells can react to and change. Brillouin light scattering offers the ability to measure mechanical properties in a non-contact and label-free manner inside of objects with high spatial resolution using light, and hence has emerged as an attractive method during the past decade.

Impulsive stimulated Brillouin microscopy for non-contact, fast mechanical investigations of hydrogels.

The mechanical properties of tissues and cells are increasingly recognized as an important feature for the understanding of pathological processes and as a diagnostic tool in biomedicine. Impulsive stimulated Brillouin scattering (ISBS) is promising to overcome shortcomings of other measurement methods such as invasiveness, low spatial resolution and long acquisition time. In this paper, we present for the first time ISBS measurements of hydrogels, which are model materials for biological samples.