Publications by authors named "Benedicte Rossi"
In the classical view, NMDA receptors (NMDARs) are located postsynaptically and play a pivotal role in excitatory transmission and synaptic plasticity. In developing cerebellar molecular layer interneurons (MLIs) however, NMDARs are known to be solely extra- or presynaptic and somewhat poorly expressed. Somatodendritic NMDARs are exclusively activated by glutamate spillover from adjacent synapses, but the mode of activation of axonal NMDARs remains unclear.
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- NMDA enhances spontaneous GABA release in developing cerebellar molecular layer interneurons, potentially through presynaptic NMDA receptors (preNMDARs) or an indirect pathway involving somato-dendritic NMDARs.
- Experiments using Ca(2+) imaging and electrophysiology showed that activating NMDA or glutamate at specific axonal spots triggered significant but localized Ca(2+) transients, sensitive to the location of the stimulation.
- The findings indicate that preNMDARs likely exist in developing MLIs and play a critical role in increasing GABA release via a Ca(2+)-induced release mechanism from presynaptic stores, which may be essential for synaptic plasticity.
Axons of cerebellar molecular layer interneurones (MLIs) bear ionotropic glutamate receptors. Here, we show that these receptors elicit cytosolic [Ca2+] transients in axonal varicosities following glutamate spillover induced by stimulation of parallel fibres (PFs). A spatial profile analysis indicates that these transients occur at the same locations when induced by PF stimulation or trains of action potentials.
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