Functional Phenotyping of Lung Mouse CD4 T Cells Using Multiparametric Flow Cytometry Analysis.

Gammaherpesviruses such as Epstein-Barr virus (EBV) are major modulators of the immune responses of their hosts. In the related study (PMID: 35857578), we investigated the role for Ly6Chi monocytes in shaping the function of effector CD4 T cells in the context of a murine gammaherpesvirus infection (Murid gammaherpesvirus 4) as a model of human EBV. In order to unravel the polyfunctional properties of CD4 T-cell subsets, we used multiparametric flow cytometry to perform intracellular staining on lung cells.